Rabbit CDK2 Polyclonal Antibody | anti-CDK2 antibody

CDK2 Antibody (T14)

Cat. Num. AAA288979

• Gene Names: CDK2; CDKN2; p33(CDK2)
• Reactivity: Human (Predicted Reactivity: Bovine, Hamster, Mouse, Rat, Xenopus)
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: CDK2, Antibody; CDK2 Antibody (T14); Cyclin-dependent kinase 2; Cell division protein kinase 2; p33 protein kinase; CDK2; CDKN2; anti-CDK2 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Hamster, Mouse, Rat, Xenopus)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This CDK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human CDK2.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 1-30
• Sequence Length: 264

• Applicable Applications for anti-CDK2 antibody: WB (Western Blot), IHC (Immunohistochemistry), ELISA
• Function: Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-Stransition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity). Phosphorylates CDK2AP2 (PubMed:12944431). Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks (PubMed:29203878).
• Cellular Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus, Cajal body. Cytoplasm. Endosome Note=Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CDK2 Antibody (T14) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Cdk2 Antibody (T14) western blot analysis in 293,Hela cell line lysates (35ug/lane).This demonstrates the hCdk2 antibody detected the hCdk2 protein (arrow).)

Related Product Information for anti-CDK2 antibody

CDK2 is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation.

Product Categories/Family for anti-CDK2 antibody

Crown Antibodies; Cancer; Cell Biology

References

Moshinsky, D.J., et al., Biochem. Biophys. Res. Commun. 310(3):1026-1031 (2003).
Chow, J.P., et al., J. Biol. Chem. 278(42):40815-40828 (2003).
O'Nions, J., et al., Oncogene 22(46):7181-7191 (2003).
Yun, J., et al., J. Biol. Chem. 278(38):36966-36972 (2003).
Izumiya, Y., et al., J. Virol. 77(17):9652-9661 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 16936528
• NCBI GeneID: 1017
• NCBI Accession #: NP_001789.2; NP_439892.2
• NCBI GenBank Nucleotide #: NM_001798.4
• UniProt Accession #: P24941; O75100; A8K7C6
• Molecular Weight: 33930
• NCBI Official Full Name: cyclin-dependent kinase 2 isoform 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 2
• NCBI Official Symbol: CDK2
• NCBI Official Synonym Symbols: CDKN2; p33(CDK2)
• NCBI Protein Information: cyclin-dependent kinase 2
• UniProt Protein Name: Cyclin-dependent kinase 2
• UniProt Gene Name: CDK2
• UniProt Synonym Gene Names: CDKN2
• UniProt Entry Name: CDK2_HUMAN