Rabbit anti-Human, Mouse CDK8 Polyclonal Antibody | anti-CDK8 antibody

CDK8 antibody (pAb)

Cat. Num. AAA60031

• Gene Names: CDK8; K35
• Reactivity: Human, Mouse
• Applications: Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Affinity Purified
• Synonyms: CDK8, Antibody; CDK8 antibody (pAb); K35; anti-CDK8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Concentration: 0.566ug/ul (varies by lot)

• Applicable Applications for anti-CDK8 antibody: WB (Western Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
• Immunogen: This antibody was raised against a peptide within the C-terminal region of human CDK8.
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

WB (Western Blot)

(CDK8 antibody (pAb) tested by Western blot. Detection of CDK8 by Western blot analysis. Lane 1: Nuclear extract of Caco-2 cells (20 ug). Lane 2: Nuclear extract of SW-48 cells (20 ug). Both were probed with CDK8 antibody (pAb) at a 1:1,000 dilution.)

ChIP (Chromatin Immunoprecipitation)

(CDK8 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from Mouse embryonic fibroblasts and 7 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify CDK8 binding sites. The image shows binding across a region of chromosome 7. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)

Related Product Information for anti-CDK8 antibody

Short Description: Our CDK8 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot; it has been shown to react with Human and Mouse samples.

Background: CDK8 (Cyclin-Dependent Kinase 8) is a component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. CDK8 phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Other studies have indicated a role in transcriptional activation in response to hypoxia.

Product Categories/Family for anti-CDK8 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4502745
• NCBI GeneID: 1024
• NCBI Accession #: NP_001251; AB_2793654
• NCBI GenBank Nucleotide #: NM_001260.1
• UniProt Accession #: P49336; Q5VUF3; Q6ISB5
• Molecular Weight: 53,156 Da
• NCBI Official Full Name: cyclin-dependent kinase 8
• NCBI Official Synonym Full Names: cyclin-dependent kinase 8
• NCBI Official Symbol: CDK8
• NCBI Official Synonym Symbols: K35
• NCBI Protein Information: cyclin-dependent kinase 8
• UniProt Protein Name: Cyclin-dependent kinase 8
• UniProt Gene Name: CDK8
• UniProt Entry Name: CDK8_HUMAN