WB (Western Blot)
(Mouse Cdkn2a Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Cdkn2a antibody detected the Cdkn2a protein (arrow).)
Rabbit anti-Mouse Cdkn2a Polyclonal Antibody | anti-Cdkn2a antibody
Mouse Cdkn2a Antibody (Center)
Gene Names
Cdkn2a; Arf; p16; MTS1; Pctr1; p19ARF; p16INK4a; p19<ARF>; ARF-INK4a; INK4a-ARF; Ink4a/Arf; p16(INK4a)
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Cdkn2a, Antibody; Mouse Cdkn2a Antibody (Center); Cyclin-dependent kinase inhibitor 2A; isoform 3; p19ARF; Cdkn2a {ECO:0000312|EMBL:AAB357701; ECO:0000312|MGI:MGI:104738}; anti-Cdkn2a antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Cdkn2a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-65 amino acids from the Central region of mouse Cdkn2a.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
39-65
Sequence Length
168
Applicable Applications for anti-Cdkn2a antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Clone Names
RB40175
Antigen Source
MOUSE
Function
Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2- induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination (By similarity). Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development. Isoform smARF may be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform is stabilized by C1QBP.
Cellular Location
Nucleus, nucleolus {ECO:0000250|UniProtKB:Q8N726, ECO:0000269|PubMed:10359817, ECO:0000269|PubMed:15567177, ECO:0000269|PubMed:15601844, ECO:0000269|PubMed:8521522}. Nucleus, nucleoplasm
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Mouse Cdkn2a Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Cdkn2a antibody detected the Cdkn2a protein (arrow).)
WB (Western Blot)
(Mouse Cdkn2a Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Cdkn2a antibody detected the Cdkn2a protein (arrow).)
Related Product Information for anti-Cdkn2a antibody
Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development.
References
Nogueira, C., et al. Oncogene 29(47):6222-6232(2010)
Fernandez-Diaz, L.C., et al. Development 137(20):3393-3403(2010)
Bennecke, M., et al. Cancer Cell 18(2):135-146(2010)
Negishi, M., et al. PLoS ONE 5 (8), E12373 (2010) :
Ulanet, D.B., et al. PLoS ONE 5 (8), E12454 (2010) :
Fernandez-Diaz, L.C., et al. Development 137(20):3393-3403(2010)
Bennecke, M., et al. Cancer Cell 18(2):135-146(2010)
Negishi, M., et al. PLoS ONE 5 (8), E12373 (2010) :
Ulanet, D.B., et al. PLoS ONE 5 (8), E12454 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19238
NCBI Official Full Name
cyclin-dependent kinase inhibitor 2A isoform 1
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 2A
NCBI Official Symbol
Cdkn2a
NCBI Official Synonym Symbols
Arf; p16; MTS1; Pctr1; p19ARF; p16INK4a; p19<ARF>; ARF-INK4a; INK4a-ARF; Ink4a/Arf; p16(INK4a)
NCBI Protein Information
cyclin-dependent kinase inhibitor 2A
UniProt Protein Name
Tumor suppressor ARF
UniProt Gene Name
Cdkn2a
UniProt Synonym Gene Names
ARF
UniProt Entry Name
ARF_MOUSE
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Product Notes
The Cdkn2a cdkn2a (Catalog #AAA285404) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 39-65. The Mouse Cdkn2a Antibody (Center) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cdkn2a can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Cdkn2a cdkn2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cdkn2a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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