ICC (Immunocytochemistry)
(Detection of human CENP-F by immunocytochemistry. Samples: Acetone-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CENP-F (Cat. No. AAA213766) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated (Cat. No. used at a dilution of 1:100.)
Rabbit anti-Human CENP-F/Mitosin Polyclonal Antibody | anti-CENP-F antibody
Rabbit anti-CENP-F/Mitosin IHC Antibody, Affinity Purified
Gene Names
CENPF; CENF; hcp-1; PRO1779
Reactivity
Human
Applications
Immunocytochemistry
Purity
Antigen Affinity Purified
Synonyms
CENP-F/Mitosin, Antibody; Rabbit anti-CENP-F/Mitosin IHC Antibody, Affinity Purified; CENP-F kinetochore protein; STROMS; PRO1779; mitosin; kinetochore protein CENPF; hcp-1; CILD31; centromere protein F, 350/400kDa; CENP-F; CENF; cell-cycle-dependent 350K nuclear protein; AH antigen; centromere protein F; Centromere protein F; anti-CENP-F antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for anti-CENP-F antibody
ICC (Immunocytochemistry)
Predicted Reactivity
Orangutan, Gorilla, Chimpanzee, Northern white-cheeked gibbon
Immunogen
Between 1700 and 1750
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
ICC (Immunocytochemistry)
(Detection of human CENP-F by immunocytochemistry. Samples: Acetone-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CENP-F (Cat. No. AAA213766) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated (Cat. No. used at a dilution of 1:100.)
ICC (Immunocytochemistry)
(Detection of human CENP-F by immunocytochemistry. Samples: Acetone-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CENP-F (Cat. No. AAA213766) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated (Cat. No. used at a dilution of 1:100.)
ICC (Immunocytochemistry)
(Detection of human CENP-F by immunocytochemistry. Samples: Acetone-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CENP-F (Cat. No. AAA213766) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated (Cat. No. used at a dilution of 1:100.)
ICC (Immunocytochemistry)
(Detection of human CENP-F by immunocytochemistry. Samples: Acetone-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-CENP-F (Cat. No. AAA213766) used at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC conjugated (Cat. No. used at a dilution of 1:100.)
Related Product Information for anti-CENP-F antibody
Centromere protein F (CENP-F) is a microtubule binding protein and component of the kinetochore. It is essential for kinetochore attachment, chromosome alignment, and the spindle checkpoint. CENP-F appears to be important to the association of the NudE-related proteins Ndel1 and Nde1 to the Lis1/dynein motor complex and their role in kinetochore function. CENP-F is a farnesylated protein and the farnesylation of CENP-F appears to be central to its role in mediating kinetochore-microtubule interactions as well as its degradation after mitosis. Recently, CENP-F expression has been correlated with markers of poor outcome in breast cancer.
Product Categories/Family for anti-CENP-F antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
367,764 Da
NCBI Official Full Name
Homo sapiens centromere protein F, 350/400kDa (CENPF), mRNA
NCBI Official Synonym Full Names
centromere protein F, 350/400kDa
NCBI Official Symbol
CENPF
NCBI Official Synonym Symbols
CENF; hcp-1; PRO1779
NCBI Protein Information
centromere protein F; AH antigen; CENP-F kinetochore protein; cell-cycle-dependent 350K nuclear protein; centromere protein F, 350/400kDa (mitosin); centromere protein F, 350/400ka (mitosin); kinetochore protein CENPF
UniProt Protein Name
Centromere protein F
UniProt Gene Name
CENPF
UniProt Synonym Gene Names
CENP-F
UniProt Entry Name
CENPF_HUMAN
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Product Notes
The CENP-F cenpf (Catalog #AAA213766) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CENP-F/Mitosin IHC Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CENP-F/Mitosin can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the CENP-F cenpf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CENP-F/Mitosin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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