WB (Western Blot)
(Western blot analysis of CENPF expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse CENPF Polyclonal Antibody | anti-CENPF antibody
CENPF Antibody
Gene Names
CENPF; CENF; hcp-1; CILD31; STROMS; PRO1779
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CENPF, Antibody; CENPF Antibody; AH antigen; Cell cycle dependent 350K nuclear protein; CENF; CENP F; CENP F kinetochore protein; CENP-F; CENPF; CENPF kinetochore protein; CENPF_HUMAN; Centromere protein F 350/400ka; Centromere protein F; Centromere protein F, 350/400kDa; CILD31; Hcp 1; Hcp1; Kinetochore protein CENP F; Kinetochore protein CENPF; Mitosin; PRO1779; STROMS; anti-CENPF antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CENPF antibody detects endogenous levels of total CENPF.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
3114
Applicable Applications for anti-CENPF antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human CENPF.
Subcellular Location
Cytoplasm, Perinuclear Region. Nucleus Matrix. Chromosome, Centromere, Kinetochore. Cytoplasm, Cytoskeleton, Spindle. Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body.
Tissue Specificity
Gradually accumulates during the cell cycle, reaching peak levels in G2 and M phase, and is rapidly degraded upon completion of mitosis.
Predicted Cross Reactivity
Pig
Similarity
Pig (82%)
Conjugation
Unconjugated
WB (Western Blot)
(Western blot analysis of CENPF expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of CENPF expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry)
(AAA323376 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA323376 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-CENPF antibody
Function: Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia.
Subunit Structure: Interacts with and STX4 (via C-terminus) (By similarity). Interacts (via N-terminus) with RBL1, RBL2 and SNAP25 (By similarity). Self-associates. Interacts with CENP-E and BUBR1 (via C-terminus). Interacts (via C-terminus) with NDE1, NDEL1 and RB1.
Post-translational Modifications: Hyperphosphorylated during mitosis.
Similarity: Belongs to the centromere protein F family.
Subunit Structure: Interacts with and STX4 (via C-terminus) (By similarity). Interacts (via N-terminus) with RBL1, RBL2 and SNAP25 (By similarity). Self-associates. Interacts with CENP-E and BUBR1 (via C-terminus). Interacts (via C-terminus) with NDE1, NDEL1 and RB1.
Post-translational Modifications: Hyperphosphorylated during mitosis.
Similarity: Belongs to the centromere protein F family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 367 kDa
Predicted: 368 kDa
Predicted: 368 kDa
NCBI Official Full Name
centromere protein F
NCBI Official Synonym Full Names
centromere protein F
NCBI Official Symbol
CENPF
NCBI Official Synonym Symbols
CENF; hcp-1; CILD31; STROMS; PRO1779
NCBI Protein Information
centromere protein F
UniProt Protein Name
Centromere protein F
UniProt Gene Name
CENPF
UniProt Synonym Gene Names
CENP-F
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Product Notes
The CENPF cenpf (Catalog #AAA323376) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CENPF Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CENPF can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CENPF cenpf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CENPF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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