IF (Immunofluorescence)
(AAA324592 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit anti-Human, Mouse CEP57 Polyclonal Antibody | anti-CEP57 antibody
CEP57 Antibody
Gene Names
CEP57; MVA2; PIG8; TSP57
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CEP57, Antibody; CEP57 Antibody; Centrosomal protein 57kDa; Centrosomal protein of 57 kDa; Cep57; Cep57 protein; CEP57_HUMAN; FGF2 interacting protein; FGF2-interacting protein; KIAA0092; MVA2; PIG8; Proliferation inducing protein 8; Testis specific protein 57; Testis-specific protein 57; Translokin; TSP57; anti-CEP57 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CEP57 antibody detects endogenous levels of total CEP57
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
491
Applicable Applications for anti-CEP57 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Nucleus. Cytoplasm. Cytoplasm > Cytoskeleton > Centrosome.
Tissue Specificity
Ubiquitous.
Predicted Cross Reactivity
Dog
Similarity
Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA324592 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA324592 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using MRPS7 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using MRPS7 antibody.)
Related Product Information for anti-CEP57 antibody
Function: Centrosomal protein which may be required for microtubule attachment to centrosomes. May act by forming ring-like structures around microtubules. Mediates nuclear translocation and mitogenic activity of the internalized growth factor FGF2, but that of FGF1.
Subunit Structure: Homodimer and homooligomer. Interacts with microtubules. Interacts with FGF2 and RAP80. Does not interact with FGF1 or FGF2 isoform 24 kDa.
Similarity: The C-terminal region mediates the interaction with microtubules and is able to nucleate and bundles microtubules in vitro.The centrosome localization domain (CLD) region mediates the localization to centrosomes and homooligomerization. Belongs to the translokin family.
Subunit Structure: Homodimer and homooligomer. Interacts with microtubules. Interacts with FGF2 and RAP80. Does not interact with FGF1 or FGF2 isoform 24 kDa.
Similarity: The C-terminal region mediates the interaction with microtubules and is able to nucleate and bundles microtubules in vitro.The centrosome localization domain (CLD) region mediates the localization to centrosomes and homooligomerization. Belongs to the translokin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 50 kD
Predicted: 58 kDa
Predicted: 58 kDa
NCBI Official Full Name
centrosomal protein of 57 kDa isoform b
NCBI Official Synonym Full Names
centrosomal protein 57
NCBI Official Symbol
CEP57
NCBI Official Synonym Symbols
MVA2; PIG8; TSP57
NCBI Protein Information
centrosomal protein of 57 kDa
UniProt Protein Name
Centrosomal protein of 57 kDa
UniProt Gene Name
CEP57
UniProt Synonym Gene Names
KIAA0092; TSP57; Cep57
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Product Notes
The CEP57 cep57 (Catalog #AAA324592) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CEP57 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CEP57 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the CEP57 cep57 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEP57, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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