FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA290619 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA290619, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Rabbit anti-Human CERS4 Polyclonal Antibody | anti-CERS4 antibody
CERS4 Antibody (N-Term)
Gene Names
CERS4; Trh1; LASS4
Reactivity
Human
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
CERS4, Antibody; CERS4 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); Ceramide synthase 4; CerS4; LAG1 longevity assurance homolog 4; CERS4; LASS4; anti-CERS4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
This CERS4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 21-51 amino acids from human CERS4.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
394
Applicable Applications for anti-CERS4 antibody
WB (Western Blot)
Function
Ceramide synthase that catalyzes formation of ceramide from sphinganine and acyl-CoA substrates, with high selectivity toward long and very-long chains (C18:0-C22:0) as acyl donor.
Cellular Location
Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9D6J1}; Multi-pass membrane protein
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA290619 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA290619, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA290619 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA290619, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
WB (Western Blot)
(All lanes : Anti-CERS4 Antibody (N-Term) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: HT-1080 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: LNCaP whole cell lysateLane 5: PC-3 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 46 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-CERS4 Antibody (N-Term) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: HT-1080 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: LNCaP whole cell lysateLane 5: PC-3 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 46 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Product Categories/Family for anti-CERS4 antibody
References
Ota T.,et al.Nat. Genet. 36:40-45(2004). Grimwood J.,et al.Nature 428:529-535(2004). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46399
NCBI Official Full Name
ceramide synthase 4
NCBI Official Synonym Full Names
ceramide synthase 4
NCBI Official Symbol
CERS4
NCBI Official Synonym Symbols
Trh1; LASS4
NCBI Protein Information
ceramide synthase 4
UniProt Protein Name
Ceramide synthase 4
UniProt Gene Name
CERS4
UniProt Synonym Gene Names
LASS4; CerS4
UniProt Entry Name
CERS4_HUMAN
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Product Notes
The CERS4 cers4 (Catalog #AAA290619) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CERS4 Antibody (N-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CERS4 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the CERS4 cers4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CERS4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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