IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CFH Antibody (Center) with A549 cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human CFH Polyclonal Antibody | anti-CFH antibody
CFH Antibody (Center)
Gene Names
CFH; FH; HF; HF1; HF2; HUS; FHL1; AHUS1; AMBP1; ARMD4; ARMS1; CFHL3
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CFH, Antibody; CFH Antibody (Center); Complement factor H; H factor 1; CFH; HF; HF1; HF2; anti-CFH antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CFH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 751-780 amino acids from the Central region of human CFH.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.287 (varies by lot)
Sequence Positions
751-780
Sequence Length
449
Applicable Applications for anti-CFH antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CFH Antibody (Center) with A549 cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CFH Antibody (Center) with A549 cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(CFH antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CFH antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(CFH antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CFH antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(CFH Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CFH antibody detected the CFH protein (arrow).)
WB (Western Blot)
(CFH Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CFH antibody detected the CFH protein (arrow).)
Related Product Information for anti-CFH antibody
This gene is a member of the Regulator of Complement
Activation (RCA) gene cluster and encodes a protein with twenty
short concensus repeat (SCR) domains. This protein is secreted into
the bloodstream and has an essential role in the regulation of
complement activation, restricting this innate defense mechanism to
microbial infections. Mutations in this gene have been associated
with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic
nephropathy. Alternate transcriptional splice variants, encoding
different isoforms, have been characterized.
Activation (RCA) gene cluster and encodes a protein with twenty
short concensus repeat (SCR) domains. This protein is secreted into
the bloodstream and has an essential role in the regulation of
complement activation, restricting this innate defense mechanism to
microbial infections. Mutations in this gene have been associated
with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic
nephropathy. Alternate transcriptional splice variants, encoding
different isoforms, have been characterized.
Product Categories/Family for anti-CFH antibody
References
Dieterich, R., et al. Infect. Immun. 78(11):4467-4476(2010)
Sofat, R., et al. Atherosclerosis 213(1):184-190(2010)
Davila, S., et al. Nat. Genet. 42(9):772-776(2010)
Scambi, C., et al. PLoS ONE 5 (8), E12162 (2010) :
Bunkenborg, J., et al. Proteomics 4(2):454-465(2004)
Sofat, R., et al. Atherosclerosis 213(1):184-190(2010)
Davila, S., et al. Nat. Genet. 42(9):772-776(2010)
Scambi, C., et al. PLoS ONE 5 (8), E12162 (2010) :
Bunkenborg, J., et al. Proteomics 4(2):454-465(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
139096
NCBI Official Full Name
complement factor H isoform a
NCBI Official Synonym Full Names
complement factor H
NCBI Official Symbol
CFH
NCBI Official Synonym Symbols
FH; HF; HF1; HF2; HUS; FHL1; AHUS1; AMBP1; ARMD4; ARMS1; CFHL3
NCBI Protein Information
complement factor H
UniProt Protein Name
Complement factor H
UniProt Gene Name
CFH
UniProt Synonym Gene Names
HF; HF1; HF2
UniProt Entry Name
CFAH_HUMAN
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Product Notes
The CFH cfh (Catalog #AAA284352) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 751-780. The CFH Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CFH can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CFH cfh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CFH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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