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WB (Western Blot)
(Anti-CFL1 Antibody (Center) at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit CFL1 Polyclonal Antibody | anti-CFL1 antibody
CFL1 Antibody (Center)
Gene Names
CFL1; CFL; cofilin; HEL-S-15
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Monkey, Pig, Rat, Chicken, Xenopus)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CFL1, Antibody; CFL1 Antibody (Center); Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin; non-muscle isoform; CFL1; CFL; anti-CFL1 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Monkey, Pig, Rat, Chicken, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CFL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the Central region of human CFL1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
70-98
Sequence Length
166
Applicable Applications for anti-CFL1 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Function
Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed:21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).
Cellular Location
Nucleus matrix. Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium {ECO:0000250|UniProtKB:P18760}. Note=Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide
Tissue Location
Widely distributed in various tissues.
Preparation and Storage
Maintain refrigerated at 2-8°C. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Anti-CFL1 Antibody (Center) at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-CFL1 Antibody (Center) at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
FCM/FACS (Flow Cytometry)
(CFL1 Antibody (Center) (AAA285656) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CFL1 Antibody (Center) (AAA285656) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of CFL1 (arrow) using rabbit polyclonal CFL1 Antibody (Center) (AAA285656). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CFL1 gene.)
WB (Western Blot)
(Western blot analysis of CFL1 (arrow) using rabbit polyclonal CFL1 Antibody (Center) (AAA285656). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CFL1 gene.)
WB (Western Blot)
(Western blot analysis of CFL1 Antibody (Center) (AAA285656) in HL-60 cell line lysates (35ug/lane). CFL1 (arrow) was detected using the purified Pab;)
WB (Western Blot)
(Western blot analysis of CFL1 Antibody (Center) (AAA285656) in HL-60 cell line lysates (35ug/lane). CFL1 (arrow) was detected using the purified Pab;)
Related Product Information for anti-CFL1 antibody
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner.
Product Categories/Family for anti-CFL1 antibody
References
Fazal,F., et.al., J. Biol. Chem. 284 (31), 21047-21056 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18502
NCBI Official Full Name
cofilin-1
NCBI Official Synonym Full Names
cofilin 1 (non-muscle)
NCBI Official Symbol
CFL1
NCBI Official Synonym Symbols
CFL; cofilin; HEL-S-15
NCBI Protein Information
cofilin-1
UniProt Protein Name
Cofilin-1
UniProt Gene Name
CFL1
UniProt Synonym Gene Names
CFL; p18
UniProt Entry Name
COF1_HUMAN
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Product Notes
The CFL1 cfl1 (Catalog #AAA285656) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 70-98. The CFL1 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Bovine, Monkey, Pig, Rat, Chicken, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's CFL1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CFL1 cfl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CFL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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