WB (Western Blot)
(Detection of human CHD1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD1 antibody AAA211358 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. CHD1 was also immunoprecipitated by rabbit anti-CHD1 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).)
Rabbit anti-Human CHD1 Polyclonal Antibody | anti-CHD1 antibody
Rabbit anti-CHD1 Antibody, Affinity Purified
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CHD1, Antibody; Rabbit anti-CHD1 Antibody, Affinity Purified; PILBOS; ATP-dependent helicase CHD1; CHD-1; chromodomain-helicase-DNA-binding protein 1; Chromodomain-helicase-DNA-binding protein 1; chromodomain helicase DNA binding protein 1; anti-CHD1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-CHD1 antibody
ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Chicken
Immunogen
Between 1660 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CHD1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD1 antibody AAA211358 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. CHD1 was also immunoprecipitated by rabbit anti-CHD1 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human CHD1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD1 antibody AAA211358 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. CHD1 was also immunoprecipitated by rabbit anti-CHD1 antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).)
ChIP (Chromatin Immunoprecipitation)
(Localization of CHD1 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CHD1 antibody AAA211358 and analyzed by DNA sequencing. The figure illustrates the peak distribution of CHD1 binding within a 3.5 Mb region of the human X chromosome as detected using anti-CHD1 antibody AAA211358. ChIP-seq validation performed by Diogenode, Denville, NJ.)
ChIP (Chromatin Immunoprecipitation)
(Localization of CHD1 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CHD1 antibody AAA211358 and analyzed by DNA sequencing. The figure illustrates the peak distribution of CHD1 binding within a 3.5 Mb region of the human X chromosome as detected using anti-CHD1 antibody AAA211358. ChIP-seq validation performed by Diogenode, Denville, NJ.)
ChIP (Chromatin Immunoprecipitation)
(ChIP-chip scatter plot of anti-CHD1 (AAA211358) enriched DNA binding sites versus input reference DNA. A. 10 ug of AAA211358 was used to immunoprecipitate chromatin from K-562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-CHD1ChIP, normal rabbit IgG showed little enrichment.)
ChIP (Chromatin Immunoprecipitation)
(ChIP-chip scatter plot of anti-CHD1 (AAA211358) enriched DNA binding sites versus input reference DNA. A. 10 ug of AAA211358 was used to immunoprecipitate chromatin from K-562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-CHD1ChIP, normal rabbit IgG showed little enrichment.)
Related Product Information for anti-CHD1 antibody
CHD1 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and functional domains: a chromodomain (chromatin organization modifier), an SNF2-like helicase/ATPase domain, and a C-terminal DNA-binding domain. CHD1 has been shown to interact with the transcriptional corepressor NCoR and histone deacetylase 1 indicating a role in transcriptional regulation. CHD1 has also been shown to interact with the Paf1 complex and Rtf1 implicating an additional role in transcriptional elongation.
Product Categories/Family for anti-CHD1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
196,590 Da
NCBI Official Full Name
chromodomain-helicase-DNA-binding protein 1
NCBI Official Synonym Full Names
chromodomain helicase DNA binding protein 1
NCBI Official Symbol
CHD1
NCBI Protein Information
chromodomain-helicase-DNA-binding protein 1
UniProt Protein Name
Chromodomain-helicase-DNA-binding protein 1
UniProt Gene Name
CHD1
UniProt Synonym Gene Names
CHD-1
UniProt Entry Name
CHD1_HUMAN
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Product Notes
The CHD1 chd1 (Catalog #AAA211358) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CHD1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHD1 can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CHD1 chd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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