WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using CHFR Antibody.)
Rabbit CHFR Polyclonal Antibody | anti-CHFR antibody
CHFR Antibody
Gene Names
CHFR; RNF116; RNF196
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CHFR, Antibody; CHFR Antibody; Checkpoint with forkhead and ring finger domains; Checkpoint with forkhead and RING finger domains protein; Chfr; CHFR_HUMAN; E3 ubiquitin protein ligase CHFR; E3 ubiquitin-protein ligase CHFR; EC 6.3.2.; FLJ10796; FLJ33629; RING finger protein 196; RNF 116; RNF 196; RNF116; RNF196; Ubiquitin ligase protein; anti-CHFR antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CHFR antibody detects endogenous levels of total CHFR
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
664
Applicable Applications for anti-CHFR antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Nucleus > PML Body.
Tissue Specificity
Ubiquitous.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Rabbit, Dog, Xenopus
Similarity
Pig (91%), Bovine (91%), Sheep (91%), Rabbit (100%), Dog (100%), Xenopus (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using CHFR Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using CHFR Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells, using CHFR antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells, using CHFR antibody.)
IF (Immunofluorescence)
(AAA325367 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA325367 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-CHFR antibody
Function: E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.
Subunit Structure: Interacts with HDAC1 and HDAC2. Interacts with PML (with sumoylated form of PML).
Post-translational Modifications: Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc finger, the protein is also covalently poly-ADP-ribosylated by PARP1.Autoubiquitinated; may regulate its cellular level. Phosphorylated by PKB. Phosphorylation may affect its E3 ligase activity.
Similarity: The PBZ-type zinc finger (also named CYR) mediates non-covalent poly(ADP-ribose)-binding. Poly(ADP-ribose)-binding is dependent on the presence of zinc and is required for its function in antephase checkpoint.The FHA domain plays a key role in the anti-proliferative properties of the protein and is involved in initiating a cell cycle arrest at G2/M. The FHA domain may be required to interact with phosphorylated proteins. Belongs to the CHFR family.
Subunit Structure: Interacts with HDAC1 and HDAC2. Interacts with PML (with sumoylated form of PML).
Post-translational Modifications: Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc finger, the protein is also covalently poly-ADP-ribosylated by PARP1.Autoubiquitinated; may regulate its cellular level. Phosphorylated by PKB. Phosphorylation may affect its E3 ligase activity.
Similarity: The PBZ-type zinc finger (also named CYR) mediates non-covalent poly(ADP-ribose)-binding. Poly(ADP-ribose)-binding is dependent on the presence of zinc and is required for its function in antephase checkpoint.The FHA domain plays a key role in the anti-proliferative properties of the protein and is involved in initiating a cell cycle arrest at G2/M. The FHA domain may be required to interact with phosphorylated proteins. Belongs to the CHFR family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 73 kD
Predicted: 74 kDa
Predicted: 74 kDa
NCBI Official Full Name
E3 ubiquitin-protein ligase CHFR isoform 1
NCBI Official Synonym Full Names
checkpoint with forkhead and ring finger domains
NCBI Official Symbol
CHFR
NCBI Official Synonym Symbols
RNF116; RNF196
NCBI Protein Information
E3 ubiquitin-protein ligase CHFR
UniProt Protein Name
E3 ubiquitin-protein ligase CHFR
UniProt Gene Name
CHFR
UniProt Synonym Gene Names
RNF196
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Product Notes
The CHFR chfr (Catalog #AAA325367) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHFR Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CHFR can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the CHFR chfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHFR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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