ICC (Immunocytochemistry)
(Immunocytochemistry of Chondroadherin in 3T3 cells with Chondroadherin antibody at 5 μg/ml.)
Rabbit Chondroadherin Polyclonal Antibody | anti-CHAD antibody
Chondroadherin Antibody
Gene Names
CHAD; SLRR4A
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot, ELISA
Purity
Affinity chromatography purified via peptide column.
Synonyms
Chondroadherin, Antibody; Chondroadherin Antibody; Chondroadherin; Cartilage leucine-rich protein; CHAD; SLRR4A; anti-CHAD antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Chondroadherin antibody is human and mouse reactive. Chondroadherin antibody is predicted to not cross-react with CHADL protein.
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
359
Applicable Applications for anti-CHAD antibody
ICC (Immunocytochemistry), WB (Western Blot), ELISA
Immunogen
Chondroadherin antibody was raised against a 17 amino acid peptide near the carboxy terminus of human Chondroadherin.
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
293 Cell Lysate
Conjugate
Unconjugated
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
ICC (Immunocytochemistry)
(Immunocytochemistry of Chondroadherin in 3T3 cells with Chondroadherin antibody at 5 μg/ml.)
ICC (Immunocytochemistry)
(Immunocytochemistry of Chondroadherin in 3T3 cells with Chondroadherin antibody at 5 μg/ml.)
WB (Western Blot)
(Western blot analysis of Chondroadherin in 3T3 cell lysate with Chondroadherin antibody at 1 μg/ml.)
WB (Western Blot)
(Western blot analysis of Chondroadherin in 3T3 cell lysate with Chondroadherin antibody at 1 μg/ml.)
Related Product Information for anti-CHAD antibody
Chondroadherin, also known as CHAD or SLRR4A, belongs to the small leucine-rich proteoglycan (SLRP) family and class IV subfamily and plays a significant role in the regulation of chondrocyte growth and proliferation (1,2). It localizes to the extracellular matrix and contains nine tandem leucine repeats as well as N-terminal and C-terminal leucine rich domains (2-4). Chondroadherin is implicated in the adhesion of fibroblasts, osteoblasts and chondrocytes, which is mediated by interactions with Integrin alpha2 beta1 (2,4,5).
Product Categories/Family for anti-CHAD antibody
References
Neame PJ, Sommarin Y, Boynton RE, et al. The structure of a 38-kDa leucine-rich protein (chondroadherin) isolated from bovine cartilage. J. Biol. Chem. 1994; 269:21547-54. Haglund L, Tillgren V, Addis L, et al. Identification and characterization of the integrin alpha2beta1 binding motif in chondroadherin mediating cell attachment. J. Biol. Chem. 2011; 286:3925-34. Mansson B, Wenglen C, Morgelin M, et al. Association of chondroadherin with collagen type II. J. Biol. Chem. 2001; 276:32883-8. Camper L, Heinegard D, and Lundgren-Akerlund E. Integrin alpha2beta1 is a receptor for the cartilage matrix protein chondroadherin. J. Cell Biol. 1997; 138:1159-67.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 38 kDa
Observed: 36 kDa
Observed: 36 kDa
NCBI Official Full Name
chondroadherin
NCBI Official Synonym Full Names
chondroadherin
NCBI Official Symbol
CHAD
NCBI Official Synonym Symbols
SLRR4A
NCBI Protein Information
chondroadherin
UniProt Protein Name
Chondroadherin
UniProt Gene Name
CHAD
UniProt Synonym Gene Names
SLRR4A
UniProt Entry Name
CHAD_HUMAN
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Product Notes
The CHAD chad (Catalog #AAA40826) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Chondroadherin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Chondroadherin can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CHAD chad for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chondroadherin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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