IHC (Immunohiostchemistry)
(CHRDL1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHRDL1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human CHRDL1 Polyclonal Antibody | anti-CHRDL1 antibody
CHRDL1 Antibody (N-term)
Gene Names
CHRDL1; CHL; MGC1; MGCN; VOPT; NRLN1; dA141H5.1
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
CHRDL1, Antibody; CHRDL1 Antibody (N-term); Chordin-like protein 1; Neuralin-1; Neurogenesin-1; Ventroptin; CHRDL1; NRLN1; anti-CHRDL1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This CHRDL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-30 amino acids from the N-terminal region of human CHRDL1.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
2-30
Sequence Length
458
Applicable Applications for anti-CHRDL1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Antagonizes the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embryonic bone formation (By similarity). May also play an important role in regulating retinal angiogenesis through modulation of BMP4 actions in endothelial cells. Plays a role during anterior segment eye development.
Cellular Location
Secreted
Tissue Location
Expressed in the developing cornea and in the eye anterior segment in addition to the retina. Differentially expressed in the fetal brain. There is high expression in cerebellum and neocortex Expressed in retinal pericytes
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(CHRDL1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHRDL1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(CHRDL1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHRDL1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(CHRDL1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHRDL1 antibody detected the CHRDL1 protein (arrow).)
WB (Western Blot)
(CHRDL1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHRDL1 antibody detected the CHRDL1 protein (arrow).)
Related Product Information for anti-CHRDL1 antibody
CHRDL1 is an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alternatively spliced transcript variants encoding different isoforms have been described.
Product Categories/Family for anti-CHRDL1 antibody
References
Fernandes, H., et al. Cells Tissues Organs (Print) 191(6):443-452(2010)
Larman, B.W., et al. J. Am. Soc. Nephrol. 20(5):1020-1031(2009)
Kane, R., et al. Mol. Vis. 14, 1138-1148 (2008) :
Kosinski, C., et al. Proc. Natl. Acad. Sci. U.S.A. 104(39):15418-15423(2007)
Gazzerro, E., et al. Rev Endocr Metab Disord 7 (1-2), 51-65 (2006) :
Larman, B.W., et al. J. Am. Soc. Nephrol. 20(5):1020-1031(2009)
Kane, R., et al. Mol. Vis. 14, 1138-1148 (2008) :
Kosinski, C., et al. Proc. Natl. Acad. Sci. U.S.A. 104(39):15418-15423(2007)
Gazzerro, E., et al. Rev Endocr Metab Disord 7 (1-2), 51-65 (2006) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52006
NCBI Official Full Name
chordin-like protein 1 isoform 1
NCBI Official Synonym Full Names
chordin-like 1
NCBI Official Symbol
CHRDL1
NCBI Official Synonym Symbols
CHL; MGC1; MGCN; VOPT; NRLN1; dA141H5.1
NCBI Protein Information
chordin-like protein 1
UniProt Protein Name
Chordin-like protein 1
UniProt Gene Name
CHRDL1
UniProt Synonym Gene Names
NRLN1
UniProt Entry Name
CRDL1_HUMAN
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Product Notes
The CHRDL1 chrdl1 (Catalog #AAA288309) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 2-30. The CHRDL1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHRDL1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CHRDL1 chrdl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHRDL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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