WB (Western Blot)
(Western blot analysis of extracts from K562 cells, using CHST1 antibody.)
Rabbit CHST1 Polyclonal Antibody | anti-CHST1 antibody
CHST1 Antibody
Gene Names
CHST1; C6ST; KSST; GST-1; KS6ST; KSGal6ST
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CHST1, Antibody; CHST1 Antibody; Carbohydrate chondroitin 6 keratan sulfotransferase 1; Carbohydrate chondroitin 6 keratan sulfotransferase; Carbohydrate keratan sulfate Gal 6 sulfotransferase; Carbohydrate keratan sulfate Gal 6 sulfotransferase 1; Carbohydrate sulfotransferase 1; Chst1; CHST1_HUMAN; EC 2.8.2.21; Galactose N acetylglucosamine N acetylglucosamine 6 O sulfotransferase 1; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1; GST 1; GST-1; Keratan sulfate Gal 6 sulfotransferase; Keratan sulfate Gal-6 sulfotransferase; KS6ST; KSGal6ST; KSST; anti-CHST1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CHST1 antibody detects endogenous levels of total CHST1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
411
Applicable Applications for anti-CHST1 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Golgi Apparatus Membrane.
Tissue Specificity
Widely expressed at low level. Expressed in brain and skeletal muscle. Expressed by high endothelial cells (HEVs) and leukocytes.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Sheep (92%), Rabbit (91%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from K562 cells, using CHST1 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from K562 cells, using CHST1 antibody.)
IF (Immunofluorescence)
(AAA324553 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA324553 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-CHST1 antibody
Function: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation.
Similarity: Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
Similarity: Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 47 kD
Predicted: 47 kDa
Predicted: 47 kDa
NCBI Official Full Name
carbohydrate sulfotransferase 1
NCBI Official Synonym Full Names
carbohydrate sulfotransferase 1
NCBI Official Symbol
CHST1
NCBI Official Synonym Symbols
C6ST; KSST; GST-1; KS6ST; KSGal6ST
NCBI Protein Information
carbohydrate sulfotransferase 1
UniProt Protein Name
Carbohydrate sulfotransferase 1
UniProt Gene Name
CHST1
UniProt Synonym Gene Names
GST-1; KS6ST; KSGal6ST; KSST
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Product Notes
The CHST1 chst1 (Catalog #AAA324553) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHST1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CHST1 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the CHST1 chst1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHST1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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