WB (Western Blot)
(Western blot analysis of lysate from human spleen tissue lysate, using CHST12 Antibody (N-term). AAA286554 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Rabbit anti-Human CHST12 Polyclonal Antibody | anti-CHST12 antibody
CHST12 Antibody (N-term)
Gene Names
CHST12; C4S-2; C4ST2; C4ST-2
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CHST12, Antibody; CHST12 Antibody (N-term); Carbohydrate sulfotransferase 12; Chondroitin 4-O-sulfotransferase 2; Chondroitin 4-sulfotransferase 2; C4ST-2; C4ST2; Sulfotransferase Hlo; CHST12; anti-CHST12 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CHST12 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 72-106 amino acids from the N-terminal region of human CHST12.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
414
Applicable Applications for anti-CHST12 antibody
ELISA, WB (Western Blot)
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysate from human spleen tissue lysate, using CHST12 Antibody (N-term). AAA286554 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
WB (Western Blot)
(Western blot analysis of lysate from human spleen tissue lysate, using CHST12 Antibody (N-term). AAA286554 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
WB (Western Blot)
(Anti-CHST12 Antibody (N-term) at 1:2000 dilution + HL-60 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-CHST12 Antibody (N-term) at 1:2000 dilution + HL-60 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-CHST12 antibody
Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Activity toward partially desulfated dermatan sulfate is however lower. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor.
Product Categories/Family for anti-CHST12 antibody
References
Hiraoka N.,et al.J. Biol. Chem. 275:20188-20196(2000).
Xia G.,et al.Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Scherer S.W.,et al.Science 300:767-772(2003).
Xia G.,et al.Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Scherer S.W.,et al.Science 300:767-772(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48414
NCBI Official Full Name
carbohydrate sulfotransferase 12
NCBI Official Synonym Full Names
carbohydrate (chondroitin 4) sulfotransferase 12
NCBI Official Symbol
CHST12
NCBI Official Synonym Symbols
C4S-2; C4ST2; C4ST-2
NCBI Protein Information
carbohydrate sulfotransferase 12
UniProt Protein Name
Carbohydrate sulfotransferase 12
UniProt Gene Name
CHST12
UniProt Synonym Gene Names
C4ST-2; C4ST2
UniProt Entry Name
CHSTC_HUMAN
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Product Notes
The CHST12 chst12 (Catalog #AAA286554) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHST12 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHST12 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CHST12 chst12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHST12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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