IHC (Immunohiostchemistry)
(Fig: 2 Immunhistochemical analysis of cIAP1 expression in formalin-fixed, paraffin-embedded human gliomas from a brain tissue microarray using at 1: 2000. A. Two tissue cores showing a gemistocytoma, grade II tumor (left) and an anaplastic glioma-glioblastoma, grade IV tumor (right). A1 and A2, higher magnifications of the gemistocytoma. A3, higher magnification of the anaplastic glioma-glioblastoma. Hematoxylin-eosin counterstain. Higher cIAP1 expression is seen in the less malignant tumor (gemistocytoma) than the more malignant anaplastic (glioma-glioblastoma) tumor.)
Rabbit cIAP-1/HIAP-2 Polyclonal Antibody | anti-cIAP-1/HIAP-2 antibody
Polyclonal Antibody to cIAP-1/HIAP-2
Gene Names
BIRC2; API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
Reactivity
HumanPredicted: Mouse
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Serum
Synonyms
cIAP-1/HIAP-2, Antibody; Polyclonal Antibody to cIAP-1/HIAP-2; BIRC2; API1; IAP2; MIHB; RNF48; C-IAP1; IAP homolog B; IAP-2; Inhibitor of apoptosis protein 2; RING finger protein 48; TNFR2-TRAF-signaling complex protein 2; Antibody to cIAP-1/HIAP-2; Anti-cIAP-1/HIAP-2 Antibody; cIAP-1/HIAP-2 Antibody; anti-cIAP-1/HIAP-2 antibody
Host
Rabbit
Reactivity
Human
Predicted: Mouse
Predicted: Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Serum
Form/Format
50 ul sera
Sequence Length
618
Applicable Applications for anti-cIAP-1/HIAP-2 antibody
WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen Information
A synthetic peptide cIAP-1/HIAP-2 protein (amino acids 158-175 PNPLNSRAVEDISSSRTN) was used as the immunogen for this antibody
Antigen
cIAP-1/HIAP-2
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
IHC (Immunohiostchemistry)
(Fig: 2 Immunhistochemical analysis of cIAP1 expression in formalin-fixed, paraffin-embedded human gliomas from a brain tissue microarray using at 1: 2000. A. Two tissue cores showing a gemistocytoma, grade II tumor (left) and an anaplastic glioma-glioblastoma, grade IV tumor (right). A1 and A2, higher magnifications of the gemistocytoma. A3, higher magnification of the anaplastic glioma-glioblastoma. Hematoxylin-eosin counterstain. Higher cIAP1 expression is seen in the less malignant tumor (gemistocytoma) than the more malignant anaplastic (glioma-glioblastoma) tumor.)
IHC (Immunohiostchemistry)
(Fig: 2 Immunhistochemical analysis of cIAP1 expression in formalin-fixed, paraffin-embedded human gliomas from a brain tissue microarray using at 1: 2000. A. Two tissue cores showing a gemistocytoma, grade II tumor (left) and an anaplastic glioma-glioblastoma, grade IV tumor (right). A1 and A2, higher magnifications of the gemistocytoma. A3, higher magnification of the anaplastic glioma-glioblastoma. Hematoxylin-eosin counterstain. Higher cIAP1 expression is seen in the less malignant tumor (gemistocytoma) than the more malignant anaplastic (glioma-glioblastoma) tumor.)
WB (Western Blot)
(Fig: 1 Western blot analysis of cIAP1 in various cancer cell lines using at 1: 2000.)
WB (Western Blot)
(Fig: 1 Western blot analysis of cIAP1 in various cancer cell lines using at 1: 2000.)
Related Product Information for anti-cIAP-1/HIAP-2 antibody
cIAP1 (HIAP2, MIHB) is a member of the family of inhibitor of apoptosis proteins (IAP). IAPs suppress mitochondria-dependent and -independent apoptosis by binding to and inhibiting caspases through their BIR domains. The IAPs, including cIAP1, have widespread tissue protein expression, with expression levels and subcellular localization patterns differing depending on the cell lineage. This antibody recognizes cIAP1, human cIAP1 is a 618 amino acid protein. In addition to at least one BIR domain, some IAP members also have a RING-type finger motif at their carboxyl-terminal. The RING finger domain of several IAPs, including cIAP2, have E3 ubiquitin ligase activity and target the degradation of Smac/DIABLO through ubqiuitination. Smac/DIABLO is a death inducer and functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis. Degradation of cell death inducers like Smac/DIABLO is thought to be a conserved mechanism by which IAPs enhance their anti-apoptotic activity, thereby promoting cell survival. The IAPs, including cIAP1, have widespread tissue protein expression, with expression levels and subcellular localization patterns differing depending on the cell lineage (see Vischioni et al. 2005 for a comprehensive study).
Product Categories/Family for anti-cIAP-1/HIAP-2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
64,098 Da
NCBI Official Full Name
Baculoviral IAP repeat-containing protein 2
NCBI Official Synonym Full Names
baculoviral IAP repeat containing 2
NCBI Official Symbol
BIRC2
NCBI Official Synonym Symbols
API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
NCBI Protein Information
baculoviral IAP repeat-containing protein 2
UniProt Protein Name
Baculoviral IAP repeat-containing protein 2
UniProt Gene Name
BIRC2
UniProt Synonym Gene Names
API1; MIHB; RNF48; hIAP-2; hIAP2
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Product Notes
The cIAP-1/HIAP-2 birc2 (Catalog #AAA78329) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to cIAP-1/HIAP-2 reacts with Human Predicted: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's cIAP-1/HIAP-2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the cIAP-1/HIAP-2 birc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "cIAP-1/HIAP-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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