<
IF (Immunofluorescence)
(Immunofluorescence Validation of cIAP in Human Lung Tissue Immunofluorescent analysis of 4% paraformaldehydefixed human lung tissue abeling cIAP with 3325 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).)
Rabbit anti-Human, Mouse cIAP Polyclonal Antibody | anti-BIRC2 antibody
cIAP Antibody
Gene Names
BIRC2; API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
cIAP Antibody is affinity chromatography purified via peptide column.
Synonyms
cIAP, Antibody; cIAP Antibody; cIAP; API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1; IAP2; Baculoviral IAP repeat-containing protein 2; C-IAP1; IAP-2; baculoviral IAP repeat-containing 2; anti-BIRC2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
cIAP Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
618
Applicable Applications for anti-BIRC2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
cIAP antibody can be used for the detection of c-IAP by Western blot at 1 to 4 ug/mL. Antibody can also be used for immunohistochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL.
Antibody validated: Western Blot in human, mouse and rat samples;Immunohistochemistry in human samples; Immunofluorescence in human samples. Allother applications and species not yet tested.
Positive Control:
( ) : Human Lung Tissue Lysate
( ) : Cell Lystae
( ) : A549 Cell Lysate
( ) : SK-N-SH Lysate
Antibody validated: Western Blot in human, mouse and rat samples;Immunohistochemistry in human samples; Immunofluorescence in human samples. Allother applications and species not yet tested.
Positive Control:
( ) : Human Lung Tissue Lysate
( ) : Cell Lystae
( ) : A549 Cell Lysate
( ) : SK-N-SH Lysate
Immunogen
Anti-cIAP antibody was raised against a synthetic peptide corresponding to 14 amino acids at the C-terminus of human c-IAP1 c-IAP antibody detects both c-IAP1 and c-IAP2.
This immunogen is located within the amino acids 540-590 of cIAP.
This immunogen is located within the amino acids 540-590 of cIAP.
Buffer
cIAP Antibody is supplied in PBS containing 0.02% sodium azide.
Homology
Predicted species reactivity based on immunogen sequence: Pig: (93%), Chicken: (92%
Preparation and Storage
cIAP antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
IF (Immunofluorescence)
(Immunofluorescence Validation of cIAP in Human Lung Tissue Immunofluorescent analysis of 4% paraformaldehydefixed human lung tissue abeling cIAP with 3325 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).)
IF (Immunofluorescence)
(Immunofluorescence Validation of cIAP in Human Lung Tissue Immunofluorescent analysis of 4% paraformaldehydefixed human lung tissue abeling cIAP with 3325 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).)
WB (Western Blot)
(Western Blot Validation in Human and Rat Tissue Lysates Loading: 15 ug of lysates per lane. Antibodies: cIAP 3325 (1 ug/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution)
WB (Western Blot)
(Western Blot Validation in Human and Rat Tissue Lysates Loading: 15 ug of lysates per lane. Antibodies: cIAP 3325 (1 ug/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution)
WB (Western Blot)
(Western Blot Validation in Human Cell Lines Loading: 15 ug of lysates per lane. Antibodies: cIAP 3325 (2 ug/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Western Blot Validation in Human Cell Lines Loading: 15 ug of lysates per lane. Antibodies: cIAP 3325 (2 ug/mL ), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
Application Data
(Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines Loading: 15 ug of lysates per lane. Antibodies: cIAP 3325 (2 ug/mL), cIAP, competitor antibody, (1 ug/mL) and betaactin 3779 (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
Application Data
(Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines Loading: 15 ug of lysates per lane. Antibodies: cIAP 3325 (2 ug/mL), cIAP, competitor antibody, (1 ug/mL) and betaactin 3779 (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
IF (Immunofluorescence)
(Immunofluorescence Validation of cIAP in Human Lung Tissue Immunofluorescent analysis of 4% paraformaldehydefixed human lung tissue abeling cIAP with 3325 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).)
IF (Immunofluorescence)
(Immunofluorescence Validation of cIAP in Human Lung Tissue Immunofluorescent analysis of 4% paraformaldehydefixed human lung tissue abeling cIAP with 3325 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).)
IHC (Immunohistochemistry)
(Immunohistochemistry Validation of cIAP in Human Lung Tissue Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-cIAP antibody (3325) at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemistry Validation of cIAP in Human Lung Tissue Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-cIAP antibody (3325) at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
WB (Western Blot)
(Western blot analysis of c-IAP in human lung lysate with c-IAP antibody at 1 (lane A), 2 (lane B), and 4 (lane C) μg/mL, respectively.)
WB (Western Blot)
(Western blot analysis of c-IAP in human lung lysate with c-IAP antibody at 1 (lane A), 2 (lane B), and 4 (lane C) μg/mL, respectively.)
Related Product Information for anti-BIRC2 antibody
cIAP Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. The two isoforms of c-IAP (c-IAP1 and c-IAP2) are structurally related to XIAP, containing 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases-3, -7. The c-IAPs can associate with the death receptor TNF-R2, and mediate the ubiquitinization of TRAF2 following the binding of TNF-α by its receptor. Omi, a negative regulator of c-IAP, inhibits its activity by catalytically cleaving c-IAP. Another negative regulator, Smac/DIABLO, acts by enhancing the auto-ubiquitization activity of c-IAP.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 70kD
Observed: 70kD
Observed: 70kD
NCBI Official Full Name
baculoviral IAP repeat-containing protein 2 isoform 1
NCBI Official Synonym Full Names
baculoviral IAP repeat containing 2
NCBI Official Symbol
BIRC2
NCBI Official Synonym Symbols
API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
NCBI Protein Information
baculoviral IAP repeat-containing protein 2; IAP-2; IAP homolog B; apoptosis inhibitor 1; RING finger protein 48; inhibitor of apoptosis protein 2; baculoviral IAP repeat-containing 2; NFR2-TRAF signalling complex protein; TNFR2-TRAF-signaling complex protein 2
UniProt Protein Name
Baculoviral IAP repeat-containing protein 2
UniProt Gene Name
BIRC2
UniProt Synonym Gene Names
API1; IAP2; MIHB; RNF48; IAP-2; hIAP-2; hIAP2
UniProt Entry Name
BIRC2_HUMAN
Similar Products
Product Notes
The BIRC2 birc2 (Catalog #AAA10954) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The cIAP Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's cIAP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). cIAP antibody can be used for the detection of c-IAP by Western blot at 1 to 4 ug/mL. Antibody can also be used for immunohistochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL.Antibody validated: Western Blot in human, mouse and rat samples;Immunohistochemistry in human samples; Immunofluorescence in human samples. Allother applications and species not yet tested. Positive Control: ( ) : Human Lung Tissue Lysate ( ) : Cell Lystae ( ) : A549 Cell Lysate ( ) : SK-N-SH Lysate. Researchers should empirically determine the suitability of the BIRC2 birc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "cIAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.