<
WB (Western Blot)
(Detection of human CIP2A by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Hep-G2, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CIP2A antibody AAA211481 (lot AAA211481-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit CIP2A Polyclonal Antibody | anti-CIP2A antibody
Rabbit anti-CIP2A Antibody, Affinity Purified
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CIP2A, Antibody; Rabbit anti-CIP2A Antibody, Affinity Purified; KIAA1524; protein CIP2A; p90; cancerous inhibitor of PP2A; cancerous inhibitor of protein phosphatase 2A; p90 autoantigen; Protein CIP2A; cell proliferation regulating inhibitor of protein phosphatase 2A; anti-CIP2A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-CIP2A antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 855 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CIP2A by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Hep-G2, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CIP2A antibody AAA211481 (lot AAA211481-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human CIP2A by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Hep-G2, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CIP2A antibody AAA211481 (lot AAA211481-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human CIP2A by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Hep-G2, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CIP2A antibody AAA211481 (lot AAA211481-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human CIP2A by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Hep-G2, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CIP2A antibody AAA211481 (lot AAA211481-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human CIP2A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CIP2A antibody AAA211481 (lot AAA211481-2) used for IP at 6 ug per reaction. CIP2A was also immunoprecipitated by a previous lot of this antibody (lot AAA211481-1). For blotting immunoprecipitated CIP2A, AAA211481 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human CIP2A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CIP2A antibody AAA211481 (lot AAA211481-2) used for IP at 6 ug per reaction. CIP2A was also immunoprecipitated by a previous lot of this antibody (lot AAA211481-1). For blotting immunoprecipitated CIP2A, AAA211481 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IHC (Immunohistochemistry)
(Detection of human CIP2A by immunohistochemistry. Sample: FFPE colon carcinoma. Antibody: Affinity purified rabbit anti-CIP2A (Cat. No. AAA211481 Lot2) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human CIP2A by immunohistochemistry. Sample: FFPE colon carcinoma. Antibody: Affinity purified rabbit anti-CIP2A (Cat. No. AAA211481 Lot2) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-CIP2A antibody
CIP2A (cancerous inhibitor of PP2A) was originally identified as p90, a cytoplasmic autoantigen found in the serum from a cancer patient. It was later described as a protein that interacted with the c-myc oncogene and functioned as an inhibitor of protein phosphatase 2A (PP2A) activity. Inhibition of PP2A phosphatase activity by CIP2A prevents c-myc phosphorylation and counteracts c-myc proteolytic degradation, thereby stabilizing c-myc and promoting cell growth and transformation. CIP2A is found to be overexpressed in some human malignancies and is thus classified as an oncogene that may be an important contributor to tumor formation.
Product Categories/Family for anti-CIP2A antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The CIP2A kiaa1524 (Catalog #AAA211481) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CIP2A Antibody, Affinity Purified reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CIP2A can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CIP2A kiaa1524 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CIP2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.