IP (Immunoprecipitation)
(CISD1 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 4 uL anti- CISD1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti- CISD1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 12kDa Observed band size :15 kDa)
Rabbit anti-Human CISD1 Polyclonal Antibody | anti-CISD1 antibody
Anti-CISD1 Antibody, Rabbit Polyclonal
Gene Names
CISD1; ZCD1; MDS029; C10orf70; mitoNEET
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
CISD1, Antibody; Anti-CISD1 Antibody, Rabbit Polyclonal; CISD1 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-C10orf70 Antibody; Anti-MDS029 Antibody; Anti-mitoNEET Antibody; Anti-ZCD1 Antibody; CDGSH iron sulfur domain 1; anti-CISD1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CISD1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CISD1 antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Conjugation
Unconjugated
Immunogen
Recombinant Human CISD1 Protein;
Preparation
Produced in rabbits immunized with purified, recombinant Human CISD1 (rh CISD1;NP_060934.1; Lys32-Thr108). CISD1 specific IgG was purified by Human CISD1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(CISD1 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 4 uL anti- CISD1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti- CISD1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 12kDa Observed band size :15 kDa)
IP (Immunoprecipitation)
(CISD1 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 4 uL anti- CISD1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti- CISD1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 12kDa Observed band size :15 kDa)
WB (Western Blot)
(Anti-CISD1 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:12 kDa Observed band size: kDa)
WB (Western Blot)
(Anti-CISD1 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:12 kDa Observed band size: kDa)
Related Product Information for anti-CISD1 antibody
Mitochondrial dysfunction is thought to play a significant role in neurodegeneration observed in Parkinson's disease (PD), the loss of mitoNEET (CISD1), an iron-sulfur containing protein that regulates mitochondrial bioenergetics, results in mitochondrial dysfunction and loss of striatal dopamine and tyrosine hydroxylase. CDGSH iron sulfur domain 1 (CISD1, also termed mitoNEET), an iron-containing outer mitochondrial membrane protein, negatively regulates ferroptotic cancer cell death. At the cellular level, CISD1 gene expression increased during human adipocyte differentiation in correlation with adipogenic genes.Thus it is a possible role of CISD1 in obesity-associated dysfunctional adipogenesis in human VAT.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12,199 Da
NCBI Official Full Name
CDGSH iron-sulfur domain-containing protein 1
NCBI Official Synonym Full Names
CDGSH iron sulfur domain 1
NCBI Official Symbol
CISD1
NCBI Official Synonym Symbols
ZCD1; MDS029; C10orf70; mitoNEET
NCBI Protein Information
CDGSH iron-sulfur domain-containing protein 1; CDGSH iron sulfur domain-containing protein 1; zinc finger CDGSH-type domain 1; zinc finger, CDGSH-type domain 1
UniProt Protein Name
CDGSH iron-sulfur domain-containing protein 1
UniProt Gene Name
CISD1
UniProt Synonym Gene Names
C10orf70; ZCD1
UniProt Entry Name
CISD1_HUMAN
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Product Notes
The CISD1 cisd1 (Catalog #AAA258527) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CISD1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CISD1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CISD1 cisd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CISD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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