Rabbit anti-Mouse CK2 alpha/CSNK2A1 Polyclonal Antibody | anti-CSNK2A1 antibody

Anti-CK2 alpha/CSNK2A1 Antibody, Rabbit Polyclonal

Cat. Num. AAA257232

• Gene Names: Csnk2a1; Csnk2a1-rs4
• Reactivity: Mouse
• Applications: Immunoprecipitation, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: CK2 alpha/CSNK2A1, Antibody; Anti-CK2 alpha/CSNK2A1 Antibody, Rabbit Polyclonal; CSNK2A1/CK2A1 Antibody, Rabbit PAb, Antigen Affinity Purified; casein kinase 2, alpha 1 polypeptide; Anti-Csnk2a1-rs4 Antibody; anti-CSNK2A1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse CSNK2A1/CK2A1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CSNK2A1 antibody: IP (Immunoprecipitation), ELISA, WB (Western Blot)
• Immunogen: Recombinant Mouse CSNK2A1/CK2A1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse CSNK2A1/CK2A1 (rM CSNK2A1/CK2A1). CSNK2A1/CK2A1 specific IgG was purified by Mouse CSNK2A1/CK2A1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-CSNK2A1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:45 kDaObserved band size:40 kDa(We are unsure as to the identity of these extra bands.))

IP (Immunoprecipitation)

(Mouse CSNK2A1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg 293T Whole Cell LysateLane D:0.5 mg Raji Whole Cell Lysate2 uL anti-Mouse CSNK2A1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CSNK2A1 rabbit polyclonal antibody,at 1:330 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 45 kDaObserved band size: 45 kDa)

Related Product Information for anti-CSNK2A1 antibody

Casein kinase II subunit alpha, also known as CK II alpha, CSNK2A1 and CK2A1, is a member of the protein kinase superfamily, Ser/Thr protein kinase family and CK2 subfamily. Casein kinase II (CSNK2A1) is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. This kinase is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. Casein kinase II (CSNK2A1) is a constitutively active, ubiquitously expressed serine/threonine protein kinase that is thought to have a regulatory function in cell proliferation, cell differentiation and apoptosis. CSNK2A1 functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alpha and alpha') in a homomeric or heteromeric conformation. Whilst the alpha-and alpha'-subunits are catalytically identical, proteins that regulate CSNK2A1, such as cdc2 and Hsp90, preferentially bind to the alpha and not the alpha'-subunit. CSNK2A1 can phosphorylate a number of key intracellular signaling proteins implicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB). CSNK2A1 is also thought to influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via the phosphorylation of Akt.

References

Schlpfer J, et al. (1997) A radiation hybrid framework map of bovine chromosome 13. Chromosome Res. 5(8): 511-9.Wirkner U, et al. (1994) The human gene (CSNK2A1) coding for the casein kinase II subunit alpha is located on chromosome 20 and contains tandemly arranged Alu repeats. Genomics. 19(2): 257-65.Wirkner U, et al. (1998) Genomic organization and promoter identification of the human protein kinase CK2 catalytic subunit alpha (CSNK2A1). Genomics. 48(1): 71-8.

NCBI and Uniprot Product Information

• NCBI GI #: 31542427
• NCBI GeneID: 12995
• NCBI Accession #: NP_031814.2
• NCBI GenBank Nucleotide #: NM_007788.3
• UniProt Accession #: Q60737; Q8R0X4
• Molecular Weight: 45,134 Da
• NCBI Official Full Name: casein kinase II subunit alpha
• NCBI Official Synonym Full Names: casein kinase 2, alpha 1 polypeptide
• NCBI Official Symbol: Csnk2a1
• NCBI Official Synonym Symbols: Csnk2a1-rs4
• NCBI Protein Information: casein kinase II subunit alpha
• UniProt Protein Name: Casein kinase II subunit alpha
• UniProt Gene Name: Csnk2a1
• UniProt Synonym Gene Names: Ckiia; CK II alpha