Rabbit anti-Human, Mouse CKII alpha' Polyclonal Antibody | anti-CKIIalpha antibody

Rabbit anti-CKII alpha' Antibody, Affinity Purified

Cat. Num. AAA210816

• Gene Names: CSNK2A2; CK2A2; CSNK2A1; CK2alpha'
• Reactivity: Human, Mouse
• Applications: Immunoprecipitation, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: CKII alpha', Antibody; Rabbit anti-CKII alpha' Antibody, Affinity Purified; casein kinase II subunit alpha'; CSNK2A1; CK2alpha'; CK II alpha'; casein kinase 2, alpha prime polypeptide; casein kinase 2 alpha'; CK2A2; Casein kinase II subunit alpha'; casein kinase 2 alpha 2; anti-CKIIalpha antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for anti-CKIIalpha antibody: IP (Immunoprecipitation), WB (Western Blot)
• Predicted Reactivity: Bovine
• Immunogen: Between 300 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human CKII alpha' by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, HeLa, Jurkat, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha' antibody (AAA210816 lot 6) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)

WB (Western Blot)

(Detection of mouse CKII alpha' by western blot. Samples: Whole cell lysate (25 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha' antibody (AAA210816 lot 6) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)

IP (Immunoprecipitation)

(Detection of human CKII alpha' by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CKII alpha' antibody (AAA210816 lot 6) used for IP at 6 ug per reaction. CKII alpha' was also immunoprecipitated by a previous lot of this antibody (AAA210816 lot 5) and a second antibody against a different epitope of CKII alpha' . For blotting immunoprecipitated CKII alpha', AAA210816 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)

Related Product Information for anti-CKIIalpha antibody

Casein kinase II (CKII) is a serine/threonine protein kinase that is required for progression through the cell cycle but whose major functions are not clearly understood. CKII is composed of a heterotetrameric complex that includes alpha and alpha-prime catalytic subunits and a beta non-catalytic subunit. Although from independent genes, CKII alpha and CKII alpha-prime can compensate for each other for some functions. However in response to ionizing radiation (IR) damage, CKII alpha and CKII alpha-prime appear to function independently in inhibiting apoptosis signaling.

Product Categories/Family for anti-CKIIalpha antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4503097
• NCBI GeneID: 1459
• NCBI Accession #: NP_001887.1
• NCBI GenBank Nucleotide #: NM_001896.2
• UniProt Accession #: P19784
• Molecular Weight: 41kDa
• NCBI Official Full Name: casein kinase II subunit alpha'
• NCBI Official Synonym Full Names: casein kinase 2 alpha 2
• NCBI Official Symbol: CSNK2A2
• NCBI Official Synonym Symbols: CK2A2; CSNK2A1; CK2alpha'
• NCBI Protein Information: casein kinase II subunit alpha'
• UniProt Protein Name: Casein kinase II subunit alpha'
• UniProt Gene Name: CSNK2A2
• UniProt Synonym Gene Names: CK2A2; CK II alpha'
• UniProt Entry Name: CSK22_HUMAN