WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells, treated with serum (20%, 15mins), using CKS1 antibody.The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse CKS1 Polyclonal Antibody | anti-CKS1 antibody
CKS1 Antibody
Gene Names
CKS1B; CKS1; ckshs1; PNAS-16; PNAS-18
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CKS1, Antibody; CKS1 Antibody; CDC2 associated protein CKS1; CDC28 protein kinase 1; CDC28 protein kinase 1B; CDC28 protein kinase regulatory subunit 1B; Cell division control protein CKS1; CKS 1; CKS-1; CKS1; CKS1_HUMAN; Cks1b; Ckshs1; Cyclin dependent kinases regulatory subunit 1; Cyclin-dependent kinases regulatory subunit 1; NB4 apoptosis/differentiation related protein; PNAS 143; PNAS 16; PNAS 18; anti-CKS1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CKS1 antibody detects endogenous levels of total CKS1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
79
Applicable Applications for anti-CKS1 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%), Xenopus (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells, treated with serum (20%, 15mins), using CKS1 antibody.The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells, treated with serum (20%, 15mins), using CKS1 antibody.The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence)
(AAA323923 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA323923 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-CKS1 antibody
Function: Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.
Subunit Structure: Forms a homohexamer that can probably bind six kinase subunits.
Similarity: Belongs to the CKS family.
Subunit Structure: Forms a homohexamer that can probably bind six kinase subunits.
Similarity: Belongs to the CKS family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 9 kD
Predicted: 10 kDa
Predicted: 10 kDa
NCBI Official Full Name
cyclin-dependent kinases regulatory subunit 1
NCBI Official Synonym Full Names
CDC28 protein kinase regulatory subunit 1B
NCBI Official Symbol
CKS1B
NCBI Official Synonym Symbols
CKS1; ckshs1; PNAS-16; PNAS-18
NCBI Protein Information
cyclin-dependent kinases regulatory subunit 1
UniProt Protein Name
Cyclin-dependent kinases regulatory subunit 1
UniProt Gene Name
CKS1B
UniProt Synonym Gene Names
CKS1; CKS-1
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Product Notes
The CKS1 cks1b (Catalog #AAA323923) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CKS1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CKS1 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the CKS1 cks1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CKS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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