WB (Western Blot)
(Western blot analysis on A549 cell lysate using Claudin 5 Antibody, The lane on the left is treated with the antigen-specific peptide.)
Rabbit Claudin 5 Polyclonal Antibody | anti-CLDN5 antibody
Claudin 5 Antibody
Gene Names
CLDN5; AWAL; BEC1; TMVCF; TMDVCF; CPETRL1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Claudin 5, Antibody; Claudin 5 Antibody; Androgen withdrawal and apoptosis induced protein RVP1 like; AWAL; BEC 1; BEC1; Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome); Claudin-5; Claudin5; CLD5_HUMAN; CLDN 5; Cldn5; CPETR L1; CPETRL 1; CPETRL1; TMDVCF; TMVCF; Transmembrane protein deleted in VCFS; Transmembrane protein deleted in velocardiofacial syndrome; anti-CLDN5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Claudin 5 antibody detects endogenous levels of total Claudin 5
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
303
Applicable Applications for anti-CLDN5 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Claudin 5
Subcellular Location
Cell Junction > Tight Junction. Cell Membrane.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis on A549 cell lysate using Claudin 5 Antibody, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis on A549 cell lysate using Claudin 5 Antibody, The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence)
(AAA320778 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA320778 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-CLDN5 antibody
Description: Claudin 5 Plays a major role in tight junction-specific obliteration of the intercellular space. Belongs to the claudin family. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ.
Function: Plays a major role in tight junction-specific obliteration of the intercellular space.
Subunit Structure: Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ (By similarity).
Similarity: Belongs to the claudin family.
Function: Plays a major role in tight junction-specific obliteration of the intercellular space.
Subunit Structure: Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ (By similarity).
Similarity: Belongs to the claudin family.
References
Yang L, Han B, Zhang Y, Bai Y, Chao J, Hu G, Yao H; Journal: Autophagy. Engagement of circular RNA HECW2 in the nonautophagic role of ATG5 implicated in the endothelial-mesenchymal transition.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 23 kDa
Predicted: 24 kDa
Predicted: 24 kDa
NCBI Official Full Name
claudin-5 isoform 1
NCBI Official Synonym Full Names
claudin 5
NCBI Official Symbol
CLDN5
NCBI Official Synonym Symbols
AWAL; BEC1; TMVCF; TMDVCF; CPETRL1
NCBI Protein Information
claudin-5
UniProt Protein Name
Claudin-5
UniProt Gene Name
CLDN5
UniProt Synonym Gene Names
AWAL; TMVCF; TMDVCF
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Product Notes
The CLDN5 cldn5 (Catalog #AAA320778) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Claudin 5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Claudin 5 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CLDN5 cldn5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Claudin 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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