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IHC (Immunohistochemistry)
(At 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit Claudin 5 Polyclonal Antibody | anti-CLDN5 antibody
Phospho-Claudin 5 (Thr207) Antibody
Gene Names
CLDN5; AWAL; BEC1; TMVCF; CPETRL1
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (90%), Bovine (89%), Rabbit (90%), Xenopus (80%)
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
Claudin 5, Antibody; Phospho-Claudin 5 (Thr207) Antibody; Androgen withdrawal and apoptosis induced protein RVP1 like; AWAL; BEC 1; BEC1; Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome); Claudin-5; Claudin5; CLD5_HUMAN; CLDN 5; Cldn5; CPETR L1; CPETRL 1; CPETRL1; TMDVCF; TMVCF; Transmembrane protein deleted in VCFS; Transmembrane protein deleted in velocardiofacial syndrome; anti-CLDN5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (90%), Bovine (89%), Rabbit (90%), Xenopus (80%)
Predicted Reactivity: Pig (90%), Bovine (89%), Rabbit (90%), Xenopus (80%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-Claudin 5 (Thr207) Antibody detects endogenous levels of Claudin 5 only when phosphorylated at Thr207.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CLDN5 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Claudin 5 around the phosphorylation site of Thr207.
Conjugation
Unconjugated
Fragment
Fab fragment
Subunit Structure
Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ (By similarity).
Similarity
Belongs to the claudin family.
Subcellular Location
Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(At 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(At 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of Phospho-Claudin 5 (Thr207 ) in lysates of MCF7 pervanadate, using Phospho-Claudin 5 (Thr207 ) Antibody(AF4307).)
WB (Western Blot)
(Western blot analysis of Phospho-Claudin 5 (Thr207 ) in lysates of MCF7 pervanadate, using Phospho-Claudin 5 (Thr207 ) Antibody(AF4307).)
WB (Western Blot)
(Western blot analysis of extracts from MCF7 treated with pervanadate, using Phospho-Claudin 5 (Thr207 ) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from MCF7 treated with pervanadate, using Phospho-Claudin 5 (Thr207 ) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
Related Product Information for anti-CLDN5 antibody
Plays a major role in tight junction-specific obliteration of the intercellular space.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,147 Da
NCBI Official Full Name
claudin-5
NCBI Official Synonym Full Names
claudin 5
NCBI Official Symbol
CLDN5
NCBI Official Synonym Symbols
AWAL; BEC1; TMVCF; CPETRL1
NCBI Protein Information
claudin-5; TMDVCF; transmembrane protein deleted in VCFS; transmembrane protein deleted in velocardiofacial syndrome
UniProt Protein Name
Claudin-5
UniProt Gene Name
CLDN5
UniProt Synonym Gene Names
AWAL; TMVCF; TMDVCF
UniProt Entry Name
CLD5_HUMAN
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Product Notes
The CLDN5 cldn5 (Catalog #AAA329406) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Claudin 5 (Thr207) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (90%), Bovine (89%), Rabbit (90%), Xenopus (80%) and may cross-react with other species as described in the data sheet. AAA Biotech's Claudin 5 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CLDN5 cldn5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Claudin 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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