IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of Claudin 7/CLDN-7 using anti-Claudin 7/CLDN-7 antibody (AAA125707).Claudin 7/CLDN-7 was detected in paraffin-embedded section of human rectal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Claudin 7/CLDN-7 Antibody (AAA125707) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Rabbit anti-Human Claudin 7/CLDN-7 Polyclonal Antibody | anti-CLDN7 antibody
Anti-Claudin 7/CLDN-7 Antibody
Gene Names
CLDN7; CLDN-7; CEPTRL2; CPETRL2; Hs.84359; claudin-1
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
Claudin 7/CLDN-7, Antibody; Anti-Claudin 7/CLDN-7 Antibody; CLDN7; CEPTRL2; CPETRL2; Claudin-7; CLDN-7; claudin 7; anti-CLDN7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for Claudin 7/CLDN-7 detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Applicable Applications for anti-CLDN7 antibody
ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
Immunogen
E Coli-derived human Claudin 7/CLDN-7 recombinant protein (Position: F92-V211).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of Claudin 7/CLDN-7 using anti-Claudin 7/CLDN-7 antibody (AAA125707).Claudin 7/CLDN-7 was detected in paraffin-embedded section of human rectal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Claudin 7/CLDN-7 Antibody (AAA125707) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of Claudin 7/CLDN-7 using anti-Claudin 7/CLDN-7 antibody (AAA125707).Claudin 7/CLDN-7 was detected in paraffin-embedded section of human rectal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Claudin 7/CLDN-7 Antibody (AAA125707) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
FCM/FACS (Flow Cytometry)
(Figure 1. Flow Cytometry analysis of CACO-2 cells using anti-Claudin 7/CLDN-7 antibody (AAA125707).Overlay histogram showing CACO-2 cells stained with AAA125707 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Claudin 7/CLDN-7 Antibody (AAA125707, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 1. Flow Cytometry analysis of CACO-2 cells using anti-Claudin 7/CLDN-7 antibody (AAA125707).Overlay histogram showing CACO-2 cells stained with AAA125707 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Claudin 7/CLDN-7 Antibody (AAA125707, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Related Product Information for anti-CLDN7 antibody
Claudin-7 is a protein that in humans is encoded by the CLDN7 gene. This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is expressed constitutively in the mammary epithelium throughout development, and might be involved in vesicle trafficking to the basolateral membrane. It is essential for NaCl homeostasis in distal nephrons. The knockout mice lacking this gene showed severe salt wasting, chronic dehydration, and growth retardation, and died within 12 days after birth. Alternatively spliced transcript variants have been found for this gene.
References
1. Coyne, C. B., Gambling, T. M., Boucher, R. C., Carson, J. L., Johnson, L. G. Role of claudin interactions in airway tight junctional permeability. Am. J. Physiol. Lung Cell. Molec. Physiol. 285: L1166-L1178, 2003.
2. Kominsky, S. L., Argani, P., Korz, D., Evron, E., Raman, V., Garrett, E., Rein, A., Sauter, G., Kallioniemi, O. -P., Sukumar, S. Loss of the tight junction protein claudin-7 correlates with histological grade in both ductal carcinoma in situ and invasive ductal carcinoma of the breast. Oncogene 22: 2021-2033, 2003.
3. Morita, K., Furuse, M., Fujimoto, K., Tsukita, S. Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands. Proc. Nat. Acad. Sci. 96: 511-516, 1999.
2. Kominsky, S. L., Argani, P., Korz, D., Evron, E., Raman, V., Garrett, E., Rein, A., Sauter, G., Kallioniemi, O. -P., Sukumar, S. Loss of the tight junction protein claudin-7 correlates with histological grade in both ductal carcinoma in situ and invasive ductal carcinoma of the breast. Oncogene 22: 2021-2033, 2003.
3. Morita, K., Furuse, M., Fujimoto, K., Tsukita, S. Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands. Proc. Nat. Acad. Sci. 96: 511-516, 1999.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,418 Da
NCBI Official Full Name
claudin-7 isoform 1
NCBI Official Synonym Full Names
claudin 7
NCBI Official Symbol
CLDN7
NCBI Official Synonym Symbols
CLDN-7; CEPTRL2; CPETRL2; Hs.84359; claudin-1
NCBI Protein Information
claudin-7; clostridium perfringens enterotoxin receptor-like 2
UniProt Protein Name
Claudin-7
UniProt Gene Name
CLDN7
UniProt Synonym Gene Names
CEPTRL2; CPETRL2; CLDN-7
UniProt Entry Name
CLD7_HUMAN
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Product Notes
The CLDN7 cldn7 (Catalog #AAA125707) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Claudin 7/CLDN-7 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Claudin 7/CLDN-7 can be used in a range of immunoassay formats including, but not limited to, ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CLDN7 cldn7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Claudin 7/CLDN-7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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