Rabbit Cleaved-PARP Polyclonal Antibody | anti-PARP antibody

Cleaved-PARP Antibody

Cat. Num. AAA328233

• Gene Names: PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Cleaved-PARP, Antibody; Cleaved-PARP Antibody; ADP-ribosyltransferase diphtheria toxin-like 1; ADPRT 1; ADPRT; ADPRT1; APOPAIN; ARTD1; NAD(+) ADP-ribosyltransferase 1; PARP-1; PARP1; PARP1_HUMAN; Poly [ADP-ribose] polymerase 1; Poly ADP ribose polymerase 1; Poly[ADP-ribose] synthase 1; PPOL; SCA1; anti-PARP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: ~1mg/ml (varies by lot)
• Sequence Length: 1014

• Applicable Applications for anti-PARP antibody: ELISA (Peptide ELISA), WB (Western Blot)
• Immunogen: The antiserum was produced against synthesized peptide derived from human PARP.
• Preparation and Storage: Store at -20°C. Stable for 14 months from date of receipt.

WB (Western Blot)

(Western blot analysis of extracts from various samples, using Cleaved-PARP (Asp214) Ab.Lane 1: Rat heart, blocked with antigen-specific peptidesLane 2: Rat heartLane 3: Mouse brainLane 4: A549 cells(serum starvation treatment)Lane 5: Raji cells(serum starvation treatment).)

WB (Western Blot)

(Western blot analysis of extracts from mouse brain, rat brain, rat spleen, using Cleaved-PARP antibody.)

WB (Western Blot)

(Western blot analysis of extracts from 293t, using Cleaved-PARP Antibody. The lane on the left was treated with blocking peptide.)

WB (Western Blot)

(Western blot analysis of extracts from rat brain, mouse brain, mouse muscle, using Cleaved-PARP antibody.)

Related Product Information for anti-PARP antibody

Description: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed:17177976, PubMed:18172500, PubMed:19344625, PubMed:19661379, PubMed:23230272). Mediates the poly(ADP-ribosyl)ation of APLF and CHFR (PubMed:17396150). Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production (PubMed:17177976). Required for PARP9 and DTX3L recruitment to DNA damage sites (PubMed:23230272). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272). Mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity (PubMed:28190768). Mediates the poly(ADP-ribosyl)ation of histones in a HPF1-dependent manner (PubMed:27067600). Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257).

NCBI and Uniprot Product Information

• NCBI GI #: 156523968
• NCBI GeneID: 142
• NCBI Accession #: NP_001609.2
• NCBI GenBank Nucleotide #: NP_001609.2
• UniProt Accession #: P09874
• Molecular Weight: Observed: 25 kDa (active); 85 kDa (N-terminal fragment); 115 kDa (pro); Predicted: 114 kDa
• NCBI Official Full Name: poly
• NCBI Official Synonym Full Names: poly(ADP-ribose) polymerase 1
• NCBI Official Symbol: PARP1
• NCBI Official Synonym Symbols: PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
• NCBI Protein Information: poly [ADP-ribose] polymerase 1
• UniProt Protein Name: Poly [ADP-ribose] polymerase 1
• UniProt Gene Name: PARP1
• UniProt Synonym Gene Names: ADPRT; PPOL; PARP-1; ARTD1; ADPRT 1
• UniProt Entry Name: PARP1_HUMAN