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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with cMet antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)
Rabbit cMet Polyclonal Antibody | anti-cMet antibody
cMet
Gene Names
Met; HGF; HGFR; Par4; c-Met; AI838057
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Immunogen affinity purified
Synonyms
cMet, Antibody; cMet; c-Met; HGF receptor; SF receptor; Hepatocyte growth factor receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; Tyrosine-protein kinase Met; anti-cMet antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified
Form/Format
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Sequence Length
1379
Applicable Applications for anti-cMet antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
Synthetic peptide within mouse cMet aa 641-690 / 1,379.
Positive control
Mouse liver tissue lysate, Mouse kidney tissue lysate, D3 cell lysate, MEF cell lysate, N2A,Hela, HepG2, mouse liver tissue, mouse spleen tissue.
Subcellular location
Secreted, membrane
Preparation and Storage
Store at 4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with cMet antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with cMet antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-cMet antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-cMet antibody. Counter stained with hematoxylin.)
ICC (Immunocytochemistry)
(ICC staining cMet in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining cMet in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot)
(Western blot analysis of cMet on different cell lysates using anti-cMet antibody at 1/1,000 dilution.Positive control:Lane 1: Mouse liver tissue lysate.Lane 2: Mouse kidney tissue lysate.Lane 3: D3 cell lysate.Lane 4: MEF cell lysate.)
WB (Western Blot)
(Western blot analysis of cMet on different cell lysates using anti-cMet antibody at 1/1,000 dilution.Positive control:Lane 1: Mouse liver tissue lysate.Lane 2: Mouse kidney tissue lysate.Lane 3: D3 cell lysate.Lane 4: MEF cell lysate.)
Related Product Information for anti-cMet antibody
c-Met (MET or MNNG HOS Transforming gene) is a proto-oncogene that encodes a protein known as hepatocyte growth factor receptor (HGFR). MET is a membrane receptor that is essential for embryonic development and wound healing. Hepatocyte growth factor (HGF) is the only known ligand of the MET receptor. MET is normally expressed by cells of epithelial origin, while expression of HGF is restricted to cells of mesenchymal origin. Upon HGF stimulation, MET induces several biological responses that collectively give rise to a program known as invasive growth. Abnormal MET activation in cancer correlates with poor prognosis, where aberrantly active MET triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). MET is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Predicted band size: 156 kDa
NCBI Official Full Name
Hepatocyte growth factor receptor
NCBI Official Synonym Full Names
met proto-oncogene
NCBI Official Symbol
Met
NCBI Official Synonym Symbols
HGF; HGFR; Par4; c-Met; AI838057
NCBI Protein Information
hepatocyte growth factor receptor
UniProt Protein Name
Hepatocyte growth factor receptor
UniProt Gene Name
Met
UniProt Synonym Gene Names
HGF receptor; SF receptor
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Product Notes
The cMet met (Catalog #AAA268375) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The cMet reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's cMet can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the cMet met for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "cMet, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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