WB (Western Blot)
(Western blot analysis of extracts from 293t, using CNOT3 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit CNOT3 Polyclonal Antibody | anti-CNOT3 antibody
CNOT3 Antibody
Gene Names
CNOT3; NOT3; LENG2; NOT3H
Reactivity
Human, Mouse, RatPredicted: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
CNOT3, Antibody; CNOT3 Antibody; CCR4 associated factor 3; CCR4-associated factor 3; CCR4-NOT transcription complex subunit 3; CNOT3; CNOT3_HUMAN; KIAA0691; LENG2; Leukocyte receptor cluster member 2; negative regulator of transcription 3, S. cerevisiae, homolog of; NOT3 (negative regulator of transcription 3, yeast) homolog; NOT3; NOT3H; anti-CNOT3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Predicted: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
CNOT3 Antibody detects endogenous levels of CNOT3.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
753
Applicable Applications for anti-CNOT3 antibody
WB (Western Blot)
Immunogen
A synthesized peptide derived from human CNOT3.
Tissue Specificity
Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from 293t, using CNOT3 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from 293t, using CNOT3 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells, using CNOT3 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells, using CNOT3 Antibody. The lane on the left was treated with blocking peptide.)
IF (Immunofluorescence)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
IF (Immunofluorescence)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Related Product Information for anti-CNOT3 antibody
Description: Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of emryonic stem (ES) cell identity.
Subunit Structure: Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. In the complex interacts directly with CNOT2. Interacts with TIP120B and NANOS2.
Similarity: Belongs to the CNOT2/3/5 family.
Subunit Structure: Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. In the complex interacts directly with CNOT2. Interacts with TIP120B and NANOS2.
Similarity: Belongs to the CNOT2/3/5 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 120 kDa
Predicted: 82 kDa
Predicted: 82 kDa
NCBI Official Full Name
CCR4-NOT transcription complex subunit 3
NCBI Official Synonym Full Names
CCR4-NOT transcription complex subunit 3
NCBI Official Symbol
CNOT3
NCBI Official Synonym Symbols
NOT3; LENG2; NOT3H
NCBI Protein Information
CCR4-NOT transcription complex subunit 3
UniProt Protein Name
CCR4-NOT transcription complex subunit 3
UniProt Gene Name
CNOT3
UniProt Synonym Gene Names
KIAA0691; LENG2; NOT3
UniProt Entry Name
CNOT3_HUMAN
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Product Notes
The CNOT3 cnot3 (Catalog #AAA328647) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CNOT3 Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Rabbit, Dog, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's CNOT3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the CNOT3 cnot3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CNOT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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