WB (Western Blot)
(Western blot analysis of Phospho-Coagulation Factor III (Ser290) in lysates of RAW264.7 TNF 20ng/ml 30', using Phospho-Coagulation Factor III (Ser290) Antibody.)
Rabbit Coagulation Factor III Polyclonal Antibody | anti-F3 antibody
Phospho-Coagulation Factor III (Ser290) Antibody
Gene Names
F3; TF; TFA; CD142
Reactivity
Human, MousePredicted: Pig, Bovine, Sheep
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
Coagulation Factor III, Antibody; Phospho-Coagulation Factor III (Ser290) Antibody; CD142; CD142 antigen; Coagulation factor III (thromboplastin tissue factor); Coagulation factor III; F3; FLJ17960; TF; TF_HUMAN; TFA; Thromboplastin; Tissue factor; anti-F3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted: Pig, Bovine, Sheep
Predicted: Pig, Bovine, Sheep
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-Coagulation Factor III (Ser290) Antibody detects endogenous levels of Coagulation Factor III only when phosphorylated at Ser290.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
238
Applicable Applications for anti-F3 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Coagulation Factor III around the phosphorylation site of Ser290.
Tissue Specificity
Lung, placenta and pancreas.
Subcellular Location
Extracellular Region or Secreted
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of Phospho-Coagulation Factor III (Ser290) in lysates of RAW264.7 TNF 20ng/ml 30', using Phospho-Coagulation Factor III (Ser290) Antibody.)
WB (Western Blot)
(Western blot analysis of Phospho-Coagulation Factor III (Ser290) in lysates of RAW264.7 TNF 20ng/ml 30', using Phospho-Coagulation Factor III (Ser290) Antibody.)
IHC (Immunohiostchemistry)
(At 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohiostchemistry)
(At 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Related Product Information for anti-F3 antibody
Description: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Subunit Structure: Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII.
Similarity: Belongs to the tissue factor family.
Subunit Structure: Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII.
Similarity: Belongs to the tissue factor family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 33 kDa
Predicted: 34 kDa
Predicted: 34 kDa
NCBI Official Full Name
tissue factor isoform 2
NCBI Official Synonym Full Names
coagulation factor III, tissue factor
NCBI Official Symbol
F3
NCBI Official Synonym Symbols
TF; TFA; CD142
NCBI Protein Information
tissue factor
UniProt Protein Name
Tissue factor
UniProt Gene Name
F3
UniProt Synonym Gene Names
TF
UniProt Entry Name
TF_HUMAN
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Product Notes
The F3 f3 (Catalog #AAA328382) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Coagulation Factor III (Ser290) Antibody reacts with Human, Mouse Predicted: Pig, Bovine, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's Coagulation Factor III can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the F3 f3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Coagulation Factor III, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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