IHC (Immunohiostchemistry)
(COL1A1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of COL1A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit COL1A1 Polyclonal Antibody | anti-COL1A1 antibody
COL1A1 Antibody (C-term)
Gene Names
COL1A1; OI1; OI2; OI3; OI4; EDSC
Reactivity
Human, Mouse (Predicted Reactivity: Bovine, Chicken, Rat)
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
COL1A1, Antibody; COL1A1 Antibody (C-term); Collagen alpha-1(I) chain; Alpha-1 type I collagen; COL1A1; anti-COL1A1 antibody
Host
Rabbit
Reactivity
Human, Mouse (Predicted Reactivity: Bovine, Chicken, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This COL1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1077-1106 amino acids from the C-terminal region of human COL1A1.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
1077-1106
Applicable Applications for anti-COL1A1 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Clone Names
RB33615
Function
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Cellular Location
Secreted, extracellular space, extracellular matrix {ECO:0000255|PROSITE-ProRule:PRU00793}
Tissue Location
Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(COL1A1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of COL1A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(COL1A1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of COL1A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(COL1A1 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the COL1A1 antibody detected the COL1A1 protein (arrow).)
WB (Western Blot)
(COL1A1 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the COL1A1 antibody detected the COL1A1 protein (arrow).)
Related Product Information for anti-COL1A1 antibody
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish].
Product Categories/Family for anti-COL1A1 antibody
References
Blades, H.Z., et al. Bone 47(5):989-994(2010) Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) : Jin, H., et al. Osteoporos Int (2010) In press : Szczesny, G., et al. Arch Orthop Trauma Surg (2010) In press : Cheung, M.S., et al. J. Bone Miner. Res. (2010) In press :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
138941
NCBI Official Full Name
collagen alpha-1(I) chain preproprotein
NCBI Official Synonym Full Names
collagen, type I, alpha 1
NCBI Official Symbol
COL1A1
NCBI Official Synonym Symbols
OI1; OI2; OI3; OI4; EDSC
NCBI Protein Information
collagen alpha-1(I) chain
UniProt Protein Name
Collagen alpha-1(I) chain
UniProt Gene Name
COL1A1
UniProt Entry Name
CO1A1_HUMAN
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Product Notes
The COL1A1 col1a1 (Catalog #AAA284271) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1077-1106. The COL1A1 Antibody (C-term) reacts with Human, Mouse (Predicted Reactivity: Bovine, Chicken, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's COL1A1 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the COL1A1 col1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COL1A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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