IF (Immunofluorescence)
(Confocal immunofluorescent analysis of COL21A1 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human COL21A1 Polyclonal Antibody | anti-COL21A1 antibody
COL21A1 Antibody (N-term)
Gene Names
COL21A1; FP633; COLA1L; dJ708F5.1; dJ682J15.1
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
COL21A1, Antibody; COL21A1 Antibody (N-term); Collagen alpha-1(XXI) chain; COL21A1; COL1AL; anti-COL21A1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This COL21A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-232 amino acids from the N-terminal region of human COL21A1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
204-232
Sequence Length
954
Applicable Applications for anti-COL21A1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of COL21A1 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of COL21A1 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(COL21A1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of COL21A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(COL21A1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of COL21A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(COL21A1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the COL21A1 antibody detected the COL21A1 protein (arrow).)
WB (Western Blot)
(COL21A1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the COL21A1 antibody detected the COL21A1 protein (arrow).)
Related Product Information for anti-COL21A1 antibody
This gene encodes the alpha chain of type XXI collagen, a
member of the FACIT collagen family (fibril-associated collagens
with interrupted helices). Type XXI collagen is localized to
tissues containing type I collagen so, like other members of this
collagen family, it may serve to maintain the integrity of the
extracellular matrix. An alternatively spliced transcript variant
has been described, but its full-length nature has yet to be
determined.
member of the FACIT collagen family (fibril-associated collagens
with interrupted helices). Type XXI collagen is localized to
tissues containing type I collagen so, like other members of this
collagen family, it may serve to maintain the integrity of the
extracellular matrix. An alternatively spliced transcript variant
has been described, but its full-length nature has yet to be
determined.
Product Categories/Family for anti-COL21A1 antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Chou, M.Y., et al. Genomics 79(3):395-401(2002)
Tuckwell, D. Matrix Biol. 21(1):63-66(2002)
Fitzgerald, J., et al. FEBS Lett. 505(2):275-280(2001)
Chou, M.Y., et al. Genomics 79(3):395-401(2002)
Tuckwell, D. Matrix Biol. 21(1):63-66(2002)
Fitzgerald, J., et al. FEBS Lett. 505(2):275-280(2001)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
99369
NCBI Official Full Name
collagen alpha-1(XXI) chain
NCBI Official Synonym Full Names
collagen, type XXI, alpha 1
NCBI Official Symbol
COL21A1
NCBI Official Synonym Symbols
FP633; COLA1L; dJ708F5.1; dJ682J15.1
NCBI Protein Information
collagen alpha-1(XXI) chain
UniProt Protein Name
Collagen alpha-1(XXI) chain
UniProt Gene Name
COL21A1
UniProt Synonym Gene Names
COL1AL
UniProt Entry Name
COLA1_HUMAN
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Product Notes
The COL21A1 col21a1 (Catalog #AAA288468) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 204-232. The COL21A1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COL21A1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the COL21A1 col21a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COL21A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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