WB (Western Blot)
(All lanes : Anti-COL4A1 Antibody (N-term) at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: DU145 whole cell lysate Lane3: Hela whole cell lysate Lane 4: HT-1080 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 161 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human COL4A1 Polyclonal Antibody | anti-COL4A1 antibody
COL4A1 Antibody (N-term)
Gene Names
COL4A1; BSVD; RATOR
Reactivity
Human
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
COL4A1, Antibody; COL4A1 Antibody (N-term); Collagen alpha-1(IV) chain; Arresten; COL4A1; anti-COL4A1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This COL4A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-54 amino acids from the N-terminal region of human COL4A1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
27-54
Sequence Length
1,318
Applicable Applications for anti-COL4A1 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Cellular Location
Secreted, extracellular space, extracellularmatrix, basement membrane {ECO:0000250|UniProtKB:P02463}
Tissue Location
Highly expressed in placenta.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-COL4A1 Antibody (N-term) at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: DU145 whole cell lysate Lane3: Hela whole cell lysate Lane 4: HT-1080 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 161 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-COL4A1 Antibody (N-term) at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: DU145 whole cell lysate Lane3: Hela whole cell lysate Lane 4: HT-1080 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 161 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Western blot analysis of lysates from Hela cell line and human placenta tissue lysate(from left to right), using COL4A1 Antibody (N-term). AAA289431 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from Hela cell line and human placenta tissue lysate(from left to right), using COL4A1 Antibody (N-term). AAA289431 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
IF (Immunofluorescence)
(Fluorescent image of U251 cells stained with COL4A1 Antibody (N-term). AAA289431 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IF (Immunofluorescence)
(Fluorescent image of U251 cells stained with COL4A1 Antibody (N-term). AAA289431 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Related Product Information for anti-COL4A1 antibody
COL4A1 is the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.
Product Categories/Family for anti-COL4A1 antibody
References
de Vries,L.S., Ann. Neurol. 65 (1), 12-18 (2009)
Mazouni,C., Br. J. Cancer 99 (1), 68-71 (2008)
Mazouni,C., Br. J. Cancer 99 (1), 68-71 (2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
collagen alpha-1(IV) chain preproprotein
NCBI Official Synonym Full Names
collagen, type IV, alpha 1
NCBI Official Symbol
COL4A1
NCBI Official Synonym Symbols
BSVD; RATOR
NCBI Protein Information
collagen alpha-1(IV) chain
UniProt Protein Name
Collagen alpha-1(IV) chain
UniProt Gene Name
COL4A1
UniProt Entry Name
CO4A1_HUMAN
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Product Notes
The COL4A1 col4a1 (Catalog #AAA289431) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 27-54. The COL4A1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COL4A1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the COL4A1 col4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COL4A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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