Rabbit anti-Human, mouse COL5A1 Polyclonal Antibody | anti-COL5A1 antibody

COL5A1 Antibody (N-term)

Cat. Num. AAA28740

• Gene Names: COL5A1; EDSC
• Reactivity: Human, mouse
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: COL5A1, Antibody; COL5A1 Antibody (N-term); Collagen alpha-1(V) chain; COL5A1; anti-COL5A1 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This COL5A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-82 amino acids from the N-terminal region of human COL5A1.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 54-82
• Sequence Length: 1,838

• Applicable Applications for anti-COL5A1 antibody: ELISA (EIA), Immunohistochemistry-P-Leica (IHC-P), Western Blot (WB)
• Application Notes: WB~~1:1000, IHC-P-Leica~~1:500
• Function: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
• Cellular Location: Secreted, extracellular space, extracellular matrix {ECO:0000255|PROSITE-ProRule:PRU00793}
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded Human heart tissue using (AAA28740) performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human uterus tissue using (AAA28740) performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)

Application Data

(Anti-COL5A1 Antibody (N-term) at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 184 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

IHC (Immunohistochemistry)

(COL5A1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COL5A1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(COL5A1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the COL5A1 antibody detected the COL5A1 protein (arrow).)

WB (Western Blot)

(COL5A1 Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the COL5A1 antibody detected the COL5A1 protein (arrow).)

Related Product Information for anti-COL5A1 antibody

COL5A1 encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. COL5A1 product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. [providedby RefSeq].

Product Categories/Family for anti-COL5A1 antibody

Signal Transduction

References

Vitart, V., et al. Hum. Mol. Genet. 19(21):4304-4311(2010)
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Posthumus, M., et al. Med Sci Sports Exerc (2010) In press :
Borck, G., et al. Am. J. Med. Genet. A 152A (8), 2090-2093 (2010) :

NCBI and Uniprot Product Information

• NCBI GI #: 89276751
• NCBI GeneID: 1289
• NCBI Accession #: NP_000084.3
• NCBI GenBank Nucleotide #: NM_000093.4
• UniProt Accession #: P20908; Q15094; Q5SUX4
• NCBI Official Full Name: collagen alpha-1(V) chain isoform 1 preproprotein
• NCBI Official Synonym Full Names: collagen, type V, alpha 1
• NCBI Official Symbol: COL5A1
• NCBI Official Synonym Symbols: EDSC
• NCBI Protein Information: collagen alpha-1(V) chain
• UniProt Protein Name: Collagen alpha-1(V) chain
• UniProt Gene Name: COL5A1
• UniProt Entry Name: CO5A1_HUMAN