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product-image-AAA127951_IHC13.jpg IHC (Immunohiostchemistry) (Figure 2. IHC analysis of Col6a2 using anti-Col6a2 antibody (AAA127951).Col6a2 was detected in a paraffin-embedded section of Zebrafish skeletal mucsle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Col6a2 Antibody (AAA127951) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

Rabbit anti-Zebrafish Col6a2 Polyclonal Antibody | anti-Col6a2 antibody

Anti-Zebrafish Col6a2 Antibody

Reactivity
Zebrafish
Purity
Immunogen affinity purified.
Synonyms
Col6a2, Antibody; Anti-Zebrafish Col6a2 Antibody; anti-Col6a2 antibody
Ordering
Host
Rabbit
Reactivity
Zebrafish
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
PredominantlyExpressed in adult forebrain.Expressed in fetal brain, lung and kidney.
Purity/Purification
Immunogen affinity purified.
Form/Format
Liquid. Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
Concentration
500ug/ml (varies by lot)
Subcellular Localization
Secreted
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Protein Function
Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
Preparation and Storage
At -20 degree C for one year, at 4 degree C for one month. Avoid repeated freezing and thawing.

IHC (Immunohiostchemistry)

(Figure 2. IHC analysis of Col6a2 using anti-Col6a2 antibody (AAA127951).Col6a2 was detected in a paraffin-embedded section of Zebrafish skeletal mucsle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Col6a2 Antibody (AAA127951) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

product-image-AAA127951_IHC13.jpg IHC (Immunohiostchemistry) (Figure 2. IHC analysis of Col6a2 using anti-Col6a2 antibody (AAA127951).Col6a2 was detected in a paraffin-embedded section of Zebrafish skeletal mucsle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Col6a2 Antibody (AAA127951) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 1. IHC analysis of Col6a2 using anti-Col6a2 antibody (AAA127951).Col6a2 was detected in a paraffin-embedded section of Zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Col6a2 Antibody (AAA127951) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

product-image-AAA127951_IHC15.jpg IHC (Immunohistochemistry) (Figure 1. IHC analysis of Col6a2 using anti-Col6a2 antibody (AAA127951).Col6a2 was detected in a paraffin-embedded section of Zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Col6a2 Antibody (AAA127951) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

NCBI and Uniprot Product Information

UniProt Accession #

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Product Notes

The Col6a2 (Catalog #AAA127951) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Zebrafish Col6a2 Antibody reacts with Zebrafish and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "Col6a2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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