IF (Immunofluorescence)
(Confocal immunofluorescent analysis of COL8A2 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human COL8A2 Polyclonal Antibody | anti-COL8A2 antibody
COL8A2 Antibody (C-term)
Gene Names
COL8A2; FECD; PPCD; FECD1; PPCD2
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
COL8A2, Antibody; COL8A2 Antibody (C-term); Collagen alpha-2(VIII) chain; Endothelial collagen; COL8A2; anti-COL8A2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This COL8A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-599 amino acids from the C-terminal region of human COL8A2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
571-599
Sequence Length
703
Applicable Applications for anti-COL8A2 antibody
WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of COL8A2 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of COL8A2 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(COL8A2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of COL8A2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(COL8A2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of COL8A2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(COL8A2 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the COL8A2 antibody detected the COL8A2 protein (arrow).)
WB (Western Blot)
(COL8A2 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the COL8A2 antibody detected the COL8A2 protein (arrow).)
Related Product Information for anti-COL8A2 antibody
This gene encodes the alpha 2 chain of type VIII collagen.
The protein is a major component of the basement membrane of the
corneal endothelium and forms homo- or heterotrimers with alpha 1
(VIII) type collagens. Defects in this gene are associated with
Fuchs endothelial corneal dystrophy and posterior polymorphous
corneal dystrophy type 2.
The protein is a major component of the basement membrane of the
corneal endothelium and forms homo- or heterotrimers with alpha 1
(VIII) type collagens. Defects in this gene are associated with
Fuchs endothelial corneal dystrophy and posterior polymorphous
corneal dystrophy type 2.
Product Categories/Family for anti-COL8A2 antibody
References
Hemadevi, B., et al. BMC Ophthalmol 10, 3 (2010) :
Afshari, N.A., et al. Invest. Ophthalmol. Vis. Sci. 50(3):1093-1097(2009)
Liskova, P., et al. Br J Ophthalmol 91(12):1717-1718(2007)
Aldave, A.J., et al. Cornea 26(8):963-965(2007)
Zhang, C., et al. Trans Am Ophthalmol Soc 104, 85-97 (2006) :
Afshari, N.A., et al. Invest. Ophthalmol. Vis. Sci. 50(3):1093-1097(2009)
Liskova, P., et al. Br J Ophthalmol 91(12):1717-1718(2007)
Aldave, A.J., et al. Cornea 26(8):963-965(2007)
Zhang, C., et al. Trans Am Ophthalmol Soc 104, 85-97 (2006) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67244
NCBI Official Full Name
collagen alpha-2(VIII) chain isoform 1
NCBI Official Synonym Full Names
collagen, type VIII, alpha 2
NCBI Official Symbol
COL8A2
NCBI Official Synonym Symbols
FECD; PPCD; FECD1; PPCD2
NCBI Protein Information
collagen alpha-2(VIII) chain
UniProt Protein Name
Collagen alpha-2(VIII) chain
UniProt Gene Name
COL8A2
UniProt Entry Name
CO8A2_HUMAN
Similar Products
Product Notes
The COL8A2 col8a2 (Catalog #AAA286992) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 571-599. The COL8A2 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COL8A2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the COL8A2 col8a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COL8A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.