Application Data
Rabbit Collagen 1, alpha 1 propeptide Polyclonal Antibody | anti-COL1A1 antibody
Anti-Collagen I alpha1 Propeptide Sequence
Gene Names
COL1A1; OI4
Reactivity
Human, Mouse, RatExpected Reactivity: Bovine, Canine, Chicken, Feline, Finch, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Sheep, Vole, Xenopus
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified rabbit serum.
Synonyms
Collagen 1, alpha 1 propeptide, Antibody; Anti-Collagen I alpha1 Propeptide Sequence; anti-COL1A1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Expected Reactivity: Bovine, Canine, Chicken, Feline, Finch, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Sheep, Vole, Xenopus
Expected Reactivity: Bovine, Canine, Chicken, Feline, Finch, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Sheep, Vole, Xenopus
Clonality
Polyclonal
Specificity
Specificity Specific for endogenous levels of the propeptide portion of the ~180 kDa collagen I alpha 1 polypeptide in human lung fibroblast extract. The antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90° C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized.
Purity/Purification
Affinity purified rabbit serum.
Form/Format
PBS
100 ul in PBS. Adequate amount of material to conduct 10-mini Western Blots.
100 ul in PBS. Adequate amount of material to conduct 10-mini Western Blots.
Sequence Length
1464
Applicable Applications for anti-COL1A1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC: 1:100
WB: 1:1000
IHC: 1:100
Immunogen
Synthetic peptide corresponding to amino acid residues specific to the human collagen 1, alpha 1 propeptide conjugated to keyhole limpet hemocyanin (KLH).
Preparation and Storage
Store at -20 degree C; stable for at least one year.
Application Data
Application Data
Related Product Information for anti-COL1A1 antibody
Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Type 1 collagen is the most abundant protein in mammals. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the first 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procollagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al.,2005)
References
• Byers PH (1989) Inherited disorders of collagen gene structure and expression. Am J Med Genet. 34(1):72-80.
• Cabral WA, Makareeva E, Colige A, Letocha AD, Ty JM, Yeowell HN, Pals G, Leikin S, Marini JC. (2005) Mutations near amino end of alpha1(I) collagen cause combined osteogenesis imperfecta/Ehlers-Danlos syndrome by interference with N-propeptide processing. J Biol Chem. 2005 May 13;280(19):19259-69.
• Cabral WA, Makareeva E, Colige A, Letocha AD, Ty JM, Yeowell HN, Pals G, Leikin S, Marini JC. (2005) Mutations near amino end of alpha1(I) collagen cause combined osteogenesis imperfecta/Ehlers-Danlos syndrome by interference with N-propeptide processing. J Biol Chem. 2005 May 13;280(19):19259-69.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
180
NCBI Official Full Name
collagen alpha-1(I) chain preproprotein
NCBI Official Synonym Full Names
collagen, type I, alpha 1
NCBI Official Symbol
COL1A1
NCBI Official Synonym Symbols
OI4
NCBI Protein Information
collagen alpha-1(I) chain; collagen alpha-1(I) chain; alpha-1 type I collagen; pro-alpha-1 collagen type 1; collagen alpha 1 chain type I; collagen alpha-1(I) chain preproprotein; collagen of skin, tendon and bone, alpha-1 chain
UniProt Protein Name
Collagen alpha-1(I) chain
UniProt Gene Name
COL1A1
UniProt Entry Name
CO1A1_HUMAN
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Product Notes
The COL1A1 col1a1 (Catalog #AAA14237) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Collagen I alpha1 Propeptide Sequence reacts with Human, Mouse, Rat Expected Reactivity: Bovine, Canine, Chicken, Feline, Finch, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Sheep, Vole, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen 1, alpha 1 propeptide can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Quality Control: Western blots performed on each lot. WB: 1:1000 IHC: 1:100. Researchers should empirically determine the suitability of the COL1A1 col1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen 1, alpha 1 propeptide, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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