FCM/FACS (Flow Cytometry)
(FACS analysis of mouse lung cells. Collagen type I antibody (biotin) detected with PE-conjugated CD45 and PE-conjugated collagen type I secondary antibody.)
Rabbit Collagen Type I Polyclonal Antibody | anti-COL1A1 antibody
Collagen Type I antibody (biotin)
Gene Names
COL1A1; OI1; OI2; OI3; OI4; EDSC
Reactivity
Reacts with Human, Bovine, Rat, and Mouse. Less than 0.1% cross-reactivity with Type II, III, IV, V, or VI Collagens.
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, ELISA
Purity
Collagen type I antibody (biotin) was purified by affinity chromatography.
Synonyms
Collagen Type I, Antibody; Collagen Type I antibody (biotin); Polyclonal Collagen Type I; Anti-Collagen Type I; OI4; Collagen type I alpha 2; Osteogenesis Imperfecta Type IV; Collagen type I alpha 1; Collagen Of Skin Tendon And Bone; Collagen Type 1; anti-COL1A1 antibody
Host
Rabbit
Reactivity
Reacts with Human, Bovine, Rat, and Mouse. Less than 0.1% cross-reactivity with Type II, III, IV, V, or VI Collagens.
Clonality
Polyclonal
Specificity
Bovine/Human collagen type I
Purity/Purification
Collagen type I antibody (biotin) was purified by affinity chromatography.
Form/Format
Supplied a lyophilized from 20mM Potassium Phosphate, 150mM Sodium Chloride, pH 7.2, with 10 mg/ml BSA and 0.01% Sodium Azide as a preservative. Reconstitute with 0.1ml of deionizd water.
Concentration
1.0 mg/ml (by UV absorbance at 280 nm) after reconstitution. (varies by lot)
Sequence Length
885
Applicable Applications for anti-COL1A1 antibody
WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA
Immunogen
Collagen Type I from human and bovine placenta
Bioactivity
Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Preparation and Storage
Store at 4°C prior to reconstitution. Stable at 4°C as an undiluted liquid.
For longer storage mix with an equal volume of glycerol and store at -20°C.
For longer storage mix with an equal volume of glycerol and store at -20°C.
FCM/FACS (Flow Cytometry)
(FACS analysis of mouse lung cells. Collagen type I antibody (biotin) detected with PE-conjugated CD45 and PE-conjugated collagen type I secondary antibody.)
FCM/FACS (Flow Cytometry)
(FACS analysis of mouse lung cells. Collagen type I antibody (biotin) detected with PE-conjugated CD45 and PE-conjugated collagen type I secondary antibody.)
IHC (Immunohiostchemistry)
(Tissue: Human Skin at pH9. Fixation: formalin fixed paraffin embedded. Collagen Type I antibody at 10 ug/mL for 1 h at RT. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).)
IHC (Immunohiostchemistry)
(Tissue: Human Skin at pH9. Fixation: formalin fixed paraffin embedded. Collagen Type I antibody at 10 ug/mL for 1 h at RT. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).)
WB (Western Blot)
(Western Blot of Collagen I antibody. Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg-transduced cell lysates. Load: 100 ug per lane. Protein staining shown below each blot depicts equal protein loading. anti-Collagen I antibody at 0.22 ug/10 ml for overnight at 4 deg C. horseradish peroxidase-conjugated rabbit secondary antibody at 1 ug/10 ml for overnight at 4 deg C. Block: TBS with 5% Non-fat milk. Size: 138.9 kDa for Collagen I.)
WB (Western Blot)
(Western Blot of Collagen I antibody. Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg-transduced cell lysates. Load: 100 ug per lane. Protein staining shown below each blot depicts equal protein loading. anti-Collagen I antibody at 0.22 ug/10 ml for overnight at 4 deg C. horseradish peroxidase-conjugated rabbit secondary antibody at 1 ug/10 ml for overnight at 4 deg C. Block: TBS with 5% Non-fat milk. Size: 138.9 kDa for Collagen I.)
Related Product Information for anti-COL1A1 antibody
Collagen is a group of naturally occurring proteins found, in nature, exclusively in animals, especially in the flesh and connective tissues of mammals. It is the main component of connective tissue, and is the most abundant protein in mammals, making up about 25% to 35% of the whole-body protein content. Type-I collagen is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is found in tendons, the endomysium of myofibrils and the organic part of bone.
Product Categories/Family for anti-COL1A1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
collagen, type I, alpha 1, isoform CRA_a
NCBI Official Synonym Full Names
collagen, type I, alpha 1
NCBI Official Symbol
COL1A1
NCBI Official Synonym Symbols
OI1; OI2; OI3; OI4; EDSC
NCBI Protein Information
collagen alpha-1(I) chain
UniProt Protein Name
Collagen alpha-1(I) chain
UniProt Gene Name
COL1A1
UniProt Entry Name
CO1A1_HUMAN
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Product Notes
The COL1A1 col1a1 (Catalog #AAA74941) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Collagen Type I antibody (biotin) reacts with Reacts with Human, Bovine, Rat, and Mouse. Less than 0.1% cross-reactivity with Type II, III, IV, V, or VI Collagens. and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen Type I can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the COL1A1 col1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen Type I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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