WB (Western Blot)
(Western blot analysis on K562 cell lysate using Collagen XIalpha 1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse Collagen XIalpha 1 Polyclonal Antibody | anti-COL11A1 antibody
Collagen XIalpha 1 Antibody
Gene Names
COL11A1; STL2; COLL6; CO11A1
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Collagen XIalpha 1, Antibody; Collagen XIalpha 1 Antibody; COBA1_HUMAN; COL11A1; COLL6; Collagen alpha 1; Collagen alpha-1(XI) chain; collagen XI alpha 1; collagen XI, alpha 1 polypeptide; collagen, type XI, alpha 1; STL2; STL3; XI chain precursor; anti-COL11A1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Collagen XIalpha 1 antibody detects endogenous levels of Collagen XIalpha 1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1767
Applicable Applications for anti-COL11A1 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Collagen XIalpha 1
Subcellular Location
Secreted > Extracellular Space > Extracellular Matrix.
Tissue Specificity
Cartilage, placenta and some tumor or virally transformed cell lines. Isoforms using exon IIA or IIB are found in the cartilage while isoforms using only exon IIB are found in the tendon.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis on K562 cell lysate using Collagen XIalpha 1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis on K562 cell lysate using Collagen XIalpha 1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2, using Collagen XIalpha 1 Antibody. Lane 1 was treated with the blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2, using Collagen XIalpha 1 Antibody. Lane 1 was treated with the blocking peptide.)
IF (Immunofluorescence)
(AAA321205 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA321205 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-COL11A1 antibody
Description: This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Mutations in this gene are associated with type II Stickler syndrome and with Marshall syndrome. Three transcript variants encoding different isoforms have been identified for this gene.
Function: May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.
Subunit Structure: Trimers composed of three different chains: alpha 1(XI), alpha 2(XI), and alpha 3(XI). Alpha 3(XI) is a post-translational modification of alpha 1(II). Alpha 1(V) can also be found instead of alpha 3(XI)=1(II).
Post-translational Modifications: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. N-glycosylated.
Similarity: The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function (By similarity). Belongs to the fibrillar collagen family.
Function: May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.
Subunit Structure: Trimers composed of three different chains: alpha 1(XI), alpha 2(XI), and alpha 3(XI). Alpha 3(XI) is a post-translational modification of alpha 1(II). Alpha 1(V) can also be found instead of alpha 3(XI)=1(II).
Post-translational Modifications: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. N-glycosylated.
Similarity: The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function (By similarity). Belongs to the fibrillar collagen family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 181 kDa
Predicted: 182 kDa
Predicted: 182 kDa
NCBI Official Full Name
collagen alpha-1(XI) chain isoform E preproprotein
NCBI Official Synonym Full Names
collagen type XI alpha 1 chain
NCBI Official Symbol
COL11A1
NCBI Official Synonym Symbols
STL2; COLL6; CO11A1
NCBI Protein Information
collagen alpha-1(XI) chain
UniProt Protein Name
Collagen alpha-1(XI) chain
UniProt Gene Name
COL11A1
UniProt Synonym Gene Names
COLL6
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Product Notes
The COL11A1 col11a1 (Catalog #AAA321205) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Collagen XIalpha 1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen XIalpha 1 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the COL11A1 col11a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen XIalpha 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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