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IHC (Immunohistochemistry)
(AAA323660 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit Collagen XVII Polyclonal Antibody | anti-COL17A1 antibody
Collagen XVII Antibody
Gene Names
COL17A1; ERED; BP180; BPA-2; BPAG2; LAD-1; BA16H23.2
Reactivity
Human, Mouse, Rat
Purity
Peptide affinty purification
Synonyms
Collagen XVII, Antibody; Collagen XVII Antibody; 180 kDa bullous pemphigoid antigen 2; Alpha 1 type XVII collagen; BA16H23.2; BP 180; BP180; BPA 2; BPAG 2; BPAG2; Bullous pemphigoid antigen 2; COL17A1; Collagen 17; Collagen alpha 1 XVII chain; Collagen alpha 1(XVII) chain; Collagen alpha1 XVII chain; Collagen type XVII alpha 1; Collagen XVII alpha 1 polypeptide; CollagenXVII; Epidermolysis bullosa junctional localisata variant; FLJ60881; KIAA0204; LAD 1; LAD1; anti-COL17A1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Collagen XVII Antibody detects endogenous levels of total Collagen XVII.
Purity/Purification
Peptide affinty purification
Form/Format
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Immunogen
A synthesized peptide derived from human Collagen XVII, corresponding to a region within N-terminal amino acids.
Preparation and Storage
Store at -20°C.
IHC (Immunohistochemistry)
(AAA323660 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA323660 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence)
(AAA323660 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA323660 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using Collagen XVII Antibody. Lane 1 was treated with the blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain, using Collagen XVII Antibody. Lane 1 was treated with the blocking peptide.)
WB (Western Blot)
(Western blot analysis of Collagen XVII using HepG2 whole cell lysates)
WB (Western Blot)
(Western blot analysis of Collagen XVII using HepG2 whole cell lysates)
Related Product Information for anti-COL17A1 antibody
May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Mol Weight: 130,180kDa
NCBI Official Full Name
collagen alpha-1(XVII) chain
NCBI Official Synonym Full Names
collagen type XVII alpha 1 chain
NCBI Official Symbol
COL17A1
NCBI Official Synonym Symbols
ERED; BP180; BPA-2; BPAG2; LAD-1; BA16H23.2
NCBI Protein Information
collagen alpha-1(XVII) chain
UniProt Protein Name
Collagen alpha-1(XVII) chain
UniProt Gene Name
COL17A1
UniProt Synonym Gene Names
BP180; BPAG2; LAD-1; 97 kDa LAD antigen; 97-LAD; LABD97
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Product Notes
The COL17A1 col17a1 (Catalog #AAA323660) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Collagen XVII Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "Collagen XVII, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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