Rabbit anti-Human Complement C9 Polyclonal Antibody | anti-C9 antibody

Anti-Complement C9 Antibody (aa181-230)

Cat. Num. AAA162350

• Gene Names: C9; C9D; ARMD15
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: Complement C9, Antibody; Anti-Complement C9 Antibody (aa181-230); Mouse Monoclonal (IgG1, k) to Human Complement C9; C9; Complement C9; Complement component 9; Complement component C9; anti-C9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: C9 Antibody detects endogenous levels of total C9 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 559

• Applicable Applications for anti-C9 antibody: WB (Western Blot), IHC (Immunohistochemistry), ELISA
• Immunogen: Raised against synthetic peptide from human C9 (AA Range: 181-230).
• Target: Complement C9
• Immunogen Species: Complement C9 antibody was raised against Human
• Epitope: aa181-230
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20°C for up to one year.

WB (Western Blot)

(Western blot of extracts from COLO cells, using C9 Antibody. The lane on the right is treated with the synthesized peptide.)

IHC (Immunohistochemistry)

(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-C9 antibody

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.

Product Categories/Family for anti-C9 antibody

Complement Component

NCBI and Uniprot Product Information

• NCBI GI #: 4502511
• NCBI GeneID: 735
• NCBI Accession #: NP_001728.1
• NCBI GenBank Nucleotide #: NM_001737.4
• UniProt Accession #: P02748
• Molecular Weight: Predicted: 63kDa
• NCBI Official Full Name: complement component C9 preproprotein
• NCBI Official Synonym Full Names: complement C9
• NCBI Official Symbol: C9
• NCBI Official Synonym Symbols: C9D; ARMD15
• NCBI Protein Information: complement component C9
• UniProt Protein Name: Complement component C9
• UniProt Gene Name: C9