WB (Western Blot)
(Western blot analysis of extracts from wild type(WT) and COMT Rabbit pAb knockdown (KD) 293T(KD) cells, using COMT Rabbit pAb (AAA282433) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.)
Rabbit anti-Human, Rat COMT Polyclonal Antibody | anti-COMT antibody
[KD Validated] COMT Rabbit pAb
Reactivity
Human, Rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
COMT, Antibody; [KD Validated] COMT Rabbit pAb; HEL-S-98n; anti-COMT antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Applicable Applications for anti-COMT antibody
WB (Western Blot)
Positive Samples
THP-1, 293T
Cellular Location
Cell membrane, Cytoplasm, Extracellular side, Single-pass type II membrane protein
Research Area
Epigenetics Nuclear Signaling, Signal Transduction, Endocrine Metabolism, Drug metabolism, Neuroscience, Neurodegenerative Diseases, Dopamine Signaling in Parkinson's Disease
Immunogen
Recombinant protein of human COMT.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Ship: Ice Pack
Ship: Ice Pack
WB (Western Blot)
(Western blot analysis of extracts from wild type(WT) and COMT Rabbit pAb knockdown (KD) 293T(KD) cells, using COMT Rabbit pAb (AAA282433) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of extracts from wild type(WT) and COMT Rabbit pAb knockdown (KD) 293T(KD) cells, using COMT Rabbit pAb (AAA282433) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of THP-1, using COMT Rabbit pAb (AAA282433) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of THP-1, using COMT Rabbit pAb (AAA282433) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.)
Related Product Information for anti-COMT antibody
Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.
Product Categories/Family for anti-COMT antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,037 Da
NCBI Official Full Name
catechol O-methyltransferase isoform MB-COMT
NCBI Official Synonym Full Names
catechol-O-methyltransferase
NCBI Official Symbol
COMT
NCBI Protein Information
catechol O-methyltransferase
UniProt Protein Name
Catechol O-methyltransferase
UniProt Gene Name
COMT
UniProt Entry Name
COMT_HUMAN
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Product Notes
The COMT comt (Catalog #AAA282433) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The [KD Validated] COMT Rabbit pAb reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's COMT can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the COMT comt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COMT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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