IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GJA5 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit anti-Human Connexin 40 Polyclonal Antibody | anti-GJA5 antibody
Connexin 40 Antibody (N-term)
Gene Names
GJA5; CX40; ATFB11
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Connexin 40, Antibody; Connexin 40 Antibody (N-term); Gap junction alpha-5 protein; Connexin-40; Cx40; GJA5; anti-GJA5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Connexin 40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-134 amino acids from the N-terminal region of human Connexin 40.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 1.98 (varies by lot)
Sequence Positions
102-134
Sequence Length
358
Applicable Applications for anti-GJA5 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GJA5 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GJA5 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of anti-GJA5 N-term Pab in Placenta lysate. GJA5 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
WB (Western Blot)
(Western blot analysis of anti-GJA5 N-term Pab in Placenta lysate. GJA5 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Related Product Information for anti-GJA5 antibody
Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell closely packed transmembrane channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. Connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 is designated alpha-1 gap junction protein, whereas CX32 and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43. Connexins have four transmembrane, three intracellular, and two extracellular regions. Different tissues express different connexins, though tissue specificities overlap, and a given tissue or cell can express several different connexins. Developmental regulation of at least some of the connexin genes has been found. Embryo implantation is regulated in part by temporally changing patterns of expression of connexins in the embryo and the maternal decidua.
Product Categories/Family for anti-GJA5 antibody
References
Dupays, L., et al., Gene 305(1):79-90 (2003).
Simon, A.M., et al., Dev. Biol. 251(2):206-220 (2002).
Cronier, L., et al., Mol. Hum. Reprod. 8(11):1005-1013 (2002).
Oviedo-Orta, E., et al., Immunology 99(4):578-590 (2000).
Gelb, B.D., et al., Genomics 39(3):409-411 (1997).
Simon, A.M., et al., Dev. Biol. 251(2):206-220 (2002).
Cronier, L., et al., Mol. Hum. Reprod. 8(11):1005-1013 (2002).
Oviedo-Orta, E., et al., Immunology 99(4):578-590 (2000).
Gelb, B.D., et al., Genomics 39(3):409-411 (1997).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40380
NCBI Official Full Name
gap junction alpha-5 protein
NCBI Official Synonym Full Names
gap junction protein, alpha 5, 40kDa
NCBI Official Symbol
GJA5
NCBI Official Synonym Symbols
CX40; ATFB11
NCBI Protein Information
gap junction alpha-5 protein
UniProt Protein Name
Gap junction alpha-5 protein
UniProt Gene Name
GJA5
UniProt Synonym Gene Names
Cx40
UniProt Entry Name
CXA5_HUMAN
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Product Notes
The GJA5 gja5 (Catalog #AAA286115) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 102-134. The Connexin 40 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Connexin 40 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GJA5 gja5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Connexin 40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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