WB (Western Blot)
(Anti-Contactin 1 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:113 kDa)
Rabbit anti-Human Contactin-1 Polyclonal Antibody | anti-CNTN1 antibody
Anti-Contactin-1 Antibody, Rabbit Polyclonal
Gene Names
CNTN1; F3; GP135; MYPCN
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
Contactin-1, Antibody; Anti-Contactin-1 Antibody, Rabbit Polyclonal; Contactin-1 Antibody, Rabbit PAb, Antigen Affinity Purified; contactin 1; Anti-CMYP12 Antibody; Anti-F3 Antibody; Anti-GP135 Antibody; Anti-MYPCN Antibody; anti-CNTN1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Contactin-1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CNTN1 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human Contactin-1 Protein ( NP_001834.2; Met1-Ser993). Contactin-1 specific IgG was purified by Human Contactin-1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
WB (Western Blot)
(Anti-Contactin 1 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:113 kDa)
WB (Western Blot)
(Anti-Contactin 1 rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:113 kDa)
IP (Immunoprecipitation)
(CNTN1 was immunoprecipitated using: Lane A:0.5 mg A549 Whole Cell Lysate 4 uL anti-CNTN1 rabbit polyclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CNTN1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 130 kDa Observed band size :150 kDa)
IP (Immunoprecipitation)
(CNTN1 was immunoprecipitated using: Lane A:0.5 mg A549 Whole Cell Lysate 4 uL anti-CNTN1 rabbit polyclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CNTN1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 130 kDa Observed band size :150 kDa)
IHC (Immunohistochemistry)
(Immunochemical staining of human Contactin-1 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human Contactin-1 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-CNTN1 antibody
Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2 (TAG-1), Contactin-3 (BIG-1), BIG-2, Contactin-5 (NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-1 is a cell surface adhesion molecule that is normally expressed by neurons and oligodendrocytes. Particularly high levels of Contactin-1 are present during brain development. Contactin-1 and Contactin-2 are differentially expressed in a number of neuronal tissues during development, and they interact with several ligands including Nr-CAM, L1, NCAM, neurocan, phosphacan, and tenascin. As a cell adhesion molecule, Contactin-1 plays a role in the formation of axon connections in the developing nervous system. It was demonstrated that Contactin-1 participates in signal pathways via its association with Contactin-associated protein (CNTNAP1), receptor protein tyrosine phosphatase beta (RPTPb) and NOTCH1. Contactin-1 is also involved in paranodal axo-glial junction formation and oligodendrocytes generation. Furthermore, studies indicated that Contactin-1 functions importantly in the invasion and metastasis of lung adenocarcinoma cells. Contactin-1 may also significantly influence the functional expression and distribution of Na+ channels in neurons.
Product Categories/Family for anti-CNTN1 antibody
References
Kazarinov. NK. e. al. (2001. Contacti. associate. wit. Na. channel. an. increase. thei. functiona. expression. . Neurosci. 2. (19):7517-25.Eckeric. C. e. al. (2006. Contacti. i. expresse. i. huma. astrocyti. glioma. an. mediate. repulsiv. effects. Glia. 53(1):1-12.S. JL. e. al. (2006. Knockdow. o. contactin-. expressio. suppresse. invasio. an. metastasi. o. lun. adenocarcinoma. Cance. researc. 6. (5):2553-61.Compto. AG. e. al. (2008. Mutation. i. contactin-1. . neura. adhesio. an. neuromuscula. junctio. protein. caus. . familia. for. o. letha. congenita. myopathy. A. . Hu. Genet. 8. (6):714-24.Mikam. T. e. al. (2009. Contactin-. i. . functiona. recepto. fo. neuroregulator. chondroiti. sulfate-E. . Bio. Chem. 284(7):4494-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70,604 Da
NCBI Official Full Name
contactin-1 isoform 1
NCBI Official Synonym Full Names
contactin 1
NCBI Official Symbol
CNTN1
NCBI Official Synonym Symbols
F3; GP135; MYPCN
NCBI Protein Information
contactin-1; glycoprotein gP135; neural cell surface protein F3
UniProt Protein Name
Contactin-1
UniProt Gene Name
CNTN1
UniProt Entry Name
CNTN1_HUMAN
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Product Notes
The CNTN1 cntn1 (Catalog #AAA259407) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Contactin-1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Contactin-1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CNTN1 cntn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Contactin-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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