WB (Western Blot)
(Detection of human Coronin 2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Coronin 2 antibody AAA211414 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Coronin 2 was also immunoprecipitated by rabbit anti-Coronin 2 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).)
Rabbit anti-Human Coronin 2 Polyclonal Antibody | anti-CORO1B antibody
Rabbit anti-Coronin 2 Antibody, Affinity Purified
Gene Names
CORO1B; CORONIN-2
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Coronin 2, Antibody; Rabbit anti-Coronin 2 Antibody, Affinity Purified; coronin, actin binding protein, 1B; coronin-1B; CORONIN-2; Coronin-1B; coronin 1B; anti-CORO1B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-CORO1B antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 439 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Coronin 2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Coronin 2 antibody AAA211414 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Coronin 2 was also immunoprecipitated by rabbit anti-Coronin 2 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).)
WB (Western Blot)
(Detection of human Coronin 2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Coronin 2 antibody AAA211414 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Coronin 2 was also immunoprecipitated by rabbit anti-Coronin 2 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human Coronin 2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Coronin 2 (Cat. No. AAA211414) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Coronin 2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Coronin 2 (Cat. No. AAA211414) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-CORO1B antibody
Coronin 2 is an F-actin binding protein that plays an important role in processes involving actin dynamics such as fibroblast migration, and phagocytosis. Coronin 2 influences actin dynamics by promoting actin depolymerization. It has been shown to interact with the Arp2/3 complex and inhibit Arp2/3-induced actin nucleation. Additionally, it can regulate motility by regulating the actin severing and disassembling activity of cofilin.
Product Categories/Family for anti-CORO1B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,235 Da
NCBI Official Full Name
coronin-1B
NCBI Official Synonym Full Names
coronin, actin binding protein, 1B
NCBI Official Symbol
CORO1B
NCBI Official Synonym Symbols
CORONIN-2
NCBI Protein Information
coronin-1B
UniProt Protein Name
Coronin-1B
UniProt Gene Name
CORO1B
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Product Notes
The CORO1B coro1b (Catalog #AAA211414) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Coronin 2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Coronin 2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CORO1B coro1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Coronin 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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