WB (Western Blot)
(Figure 2 WB Validation of of Spike 156-157EF Antibodies with SARS-CoV-2 Delta Variant Spike S1 ProteinLoading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.617.2). Detection Antibodies: SARS-CoV-2 Spike 156-157EF antibody, 1 μg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr. SARS-CoV-2 Spike 156-157EF antibody detects spike S1 protein of WT, but not Delta variant.)
Rabbit COVID-19 Spike 156-157EF Coronavirus Polyclonal Antibody | anti-COVID-19 antibody
SARS-CoV-2 (COVID-19) Spike 156-157EF Antibody
Applications
Western Blot, ELISA
Purity
Anti-SARS-CoV-2 Spike 156-157EF antibody is affinity chromatography purified via peptide column.
Synonyms
COVID-19 Spike 156-157EF Coronavirus, Antibody; SARS-CoV-2 (COVID-19) Spike 156-157EF Antibody; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike 156-157EF; anti-COVID-19 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Anti-SARS-CoV-2 Spike 156-157EF antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Applicable Applications for anti-COVID-19 antibody
WB (Western Blot), ELISA
Homology
Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (38.5%)
Imunogen
Anti-SARS-CoV-2 Spike 156-157EF antibody was raised against a peptide corresponding to 13 amino acids near the amino terminal domain of SARS-CoV-2 Spike protein.
The immunogen is located within 130-180 amino acids of SARS-CoV-2 Spike protein.
The immunogen is located within 130-180 amino acids of SARS-CoV-2 Spike protein.
Conjugation
Unconjugated
Buffer
SARS-CoV-2 Spike P26S antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SARS-CoV-2 Spike 156-157EF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
WB (Western Blot)
(Figure 2 WB Validation of of Spike 156-157EF Antibodies with SARS-CoV-2 Delta Variant Spike S1 ProteinLoading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.617.2). Detection Antibodies: SARS-CoV-2 Spike 156-157EF antibody, 1 μg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr. SARS-CoV-2 Spike 156-157EF antibody detects spike S1 protein of WT, but not Delta variant.)
WB (Western Blot)
(Figure 2 WB Validation of of Spike 156-157EF Antibodies with SARS-CoV-2 Delta Variant Spike S1 ProteinLoading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.617.2). Detection Antibodies: SARS-CoV-2 Spike 156-157EF antibody, 1 μg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubated at RT for 1 hr. SARS-CoV-2 Spike 156-157EF antibody detects spike S1 protein of WT, but not Delta variant.)
Application Data
(Figure 1 SARS-Cov-2 Spike 156-157EF Antibodies Specifically Do Not Recognize Delta Variant Spike S1 Protein in an ELISACoating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike 1566-157EF antibody, 1 μg/mL,, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.)
Application Data
(Figure 1 SARS-Cov-2 Spike 156-157EF Antibodies Specifically Do Not Recognize Delta Variant Spike S1 Protein in an ELISACoating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike 1566-157EF antibody, 1 μg/mL,, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.)
Related Product Information for anti-COVID-19 antibody
SARS-CoV-2 delta variant, a variant of concern (VOC), known as B.1.617.2, was detected in India in October of 2020. However, it rapidly spread all over of the world and now it is the dominant variant in the world, which account for more than 99% of the cases. This variant carries at least 13 mutations in spike protein across the sub lineages, including L452R, D614G, P681R and K417N, which can increase the affinity to the human ACE2 receptor. Enhanced transmission of the Delta variant was observed globally, which is at least 2.5 times more contagious as the other variants. The Delta variant affects the effectiveness of COVID19 vaccine and is resistant to neutralization to some extent.
Product Categories/Family for anti-COVID-19 antibody
NCBI and Uniprot Product Information
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Product Notes
The COVID-19 (Catalog #AAA40954) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's COVID-19 Spike 156-157EF Coronavirus can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID-19 Spike 156-157EF Coronavirus, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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