Rabbit COVID 19 Spike RBD Coronavirus Polyclonal Antibody | anti-COVID-19 antibody

SARS-CoV-2 (COVID-19) Spike RBD Antibody

Cat. Num. AAA11030

• Gene Names: S; spike glycoprotein
• Reactivity: Virus
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: SARS-CoV-2 (COVID-19) Spike RBD antibody is affinity chromatography purified via peptide column.
• Synonyms: COVID 19 Spike RBD Coronavirus, Antibody; SARS-CoV-2 (COVID-19) Spike RBD Antibody; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike RBD; Receptor Binding Domain; anti-COVID-19 antibody

• Host: Rabbit
• Reactivity: Virus
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: SARS-CoV-2 (COVID-19) Spike RBD antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; SARS-CoV-2 (COVID-19) Spike RBD antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 1273

• Applicable Applications for anti-COVID-19 antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: WB: 2-5ug/mL; IF: 20ug/mL.; ; Antibody validated: Western Blot in human samples; Immunofluorescence in human samples. SARS-CoV-2 (COVID-19) Spike RBD Antibody can be used for the detection of SARS-CoV-2 (COVID-19) Spike protein in ELISA. It will detect 4ng of free peptide at 1ug/mL. All other applications and species not yet tested.; ; Optimal dilutions for each application to be determined by researcher.
• Immunogen: Anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Spike antibody was raised against a peptide corresponding to 19 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19, 2019-nCoV) Spike glycoprotein RBD.; The immunogen is located within the last 50 amino acids of SARS-CoV-2 (COVID-19, 2019-nCoV) Spike protein RBD.
• Homology: Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (63%)
• Preparation and Storage: Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

IHC (Immunohistochemistry)

(Immunohistochemistry Validation of SARSCoV-2 (COVID-19) Spike RBD in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Spike RBD antibody (AAA11030, 0.5 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Spike RBD protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.)

ELISA

(Coating Antigen: SARS-CoV-2 full length spike proteins, including WT, UK variant (B.1.1.7), SA variant (B.1.135) and Brazil (P.1). Dilution: 10-3000 ng/mL. Incubate at 4 °C overnight.Detection Antibodies: SARS-CoV-2 Spike RBD Antibody, (AAA11030), 2 µg/mL, incubate at RT for 1 hr.Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr.Immunogen region of antibody (AAA11030) includes site 501N that was mutated in all three variants. 9087 has low binding affinity for all three variants as compared to WT.)

ELISA

(Validation with SARS-CoV-2 (COVID-19) Spike Recombinant Protein Antibodies: SARS-CoV-2 (COVID-19) Spike antibody, AAA11030 (1 ug/mL). A direct ELISA was performed using SARS-CoV-2 (COVID-19) Spike S1 recombinant protein as coating antigen and the anti-SARS-CoV-2 (COVID-19) Spike RBD antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 16 ng/mL to 2000ng/mL.)

WB (Western Blot)

(Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike RBD recombinant protein, 10-303. Antibodies: SARS-CoV-2 (COVID-19) Spike RBD, 9087, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL and Lane 2: 2 µg/mL.)

Related Product Information for anti-COVID-19 antibody

Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019-20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a Spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Coronavirus invades cells through Spike (S) glycoproteins, a class I fusion protein. It is the major viral surface protein that coronavirus uses to bind to the human cell surface receptor. It also mediates the fusion of host and viral cell membrane, allowing the virus to enter human cells and begin infection (3). The spike protein is the major target for neutralizing antibodies and vaccine development (4). The protein modeling suggests that there is strong interaction between Spike protein receptor-binding domain and its host receptor angiotensin-converting enzyme 2 (ACE2), which regulate both the cross-species and human-to-human transmissions of COVID-19 (5). The recent study has shown that the SARS-CoV-2 spike protein binds ACE2 with higher affinity than SARS-CoV spike protein (6).

Product Categories/Family for anti-COVID-19 antibody

Infectious Disease

References

1) Gorbalenya. bioRxiv: 2020.
2) Hui et al. Int J Infect Dis. 2020;91:264-266.
3) Belouzard et al. Viruses. 2012;4(6):1011-33.
4) Lee et al. J Virol. 2006;80(8):4079-87.
5) Wan et al. J Virol. 2020.
6) Wrapp et al. Science. 2020.

NCBI and Uniprot Product Information

• NCBI GI #: 1791269090
• NCBI GeneID: 43740568
• NCBI Official Full Name: surface glycoprotein
• NCBI Official Symbol: S
• NCBI Official Synonym Symbols: spike glycoprotein
• NCBI Protein Information: surface glycoprotein