Rabbit COX IV Polyclonal Antibody | anti-COXIV antibody

[KD Validated] COX IV Rabbit pAb

Cat. Num. AAA28444

• Gene Names: COX4I1; COX4; COXIV; COX4-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
• Purity: Affinity purification
• Synonyms: COX IV, Antibody; [KD Validated] COX IV Rabbit pAb; COX4; COXIV; COX4-1; COXIV-1; MC4DN16; COX IV-1; anti-COXIV antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.05% proclin300,50% glycerol,pH7.3.

• Applicable Applications for anti-COXIV antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: WB: 1:100-1:500; IHC-P: 1:50-1:200; IF/ICC: 1:50-1:200
• Positive Samples: 293T (KD), HepG2, Mouse heart, Rat heart
• Cellular Location: Mitochondrion inner membrane
• Research Area: Cancer, Signal Transduction, Endocrine Metabolism, Mitochondrial metabolism, Mitochondrial markers, Oxidative phosphorylation, Neuroscience, Neurodegenerative Diseases
• Immunogen: Recombinant protein of human COX IV.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.; Ship: Ice Pack

IF (Immunofluorescence)

(Immunofluorescence analysis of HepG2 cells using [KD Validated] COX IV Rabbit pAb (AAA28444) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of HeLa cells using [KD Validated] COX IV Rabbit pAb (AAA28444) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded mouse testis using [KD Validated] COX IV Rabbit pAb (AAA28444) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IF (Immunofluorescence)

(Immunohistochemistry analysis of paraffin-embedded human liver using [KD Validated] COX IV Rabbit pAb (AAA28444) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using [KD Validated] COX IV Rabbit pAb (AAA28444) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot)

(Western blot analysis of extracts from wild type(WT) and COX IVknockdown (KD) 293T(KD) cells, using COX IV antibody (AAA28444) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.)

WB (Western Blot)

(Western blot analysis of various lysates, using COX IV antibody (AAA28444) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.)

Related Product Information for anti-COXIV antibody

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Categories/Family for anti-COXIV antibody

Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4502981
• NCBI GeneID: 1327
• NCBI Accession #: NP_001852.1
• NCBI GenBank Nucleotide #: NM_001861.3
• UniProt Accession #: P13073; Q6P666; B2R4J2; D3DUM7
• Molecular Weight: 19,577 Da
• NCBI Official Full Name: cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• NCBI Official Synonym Full Names: cytochrome c oxidase subunit IV isoform 1
• NCBI Official Symbol: COX4I1
• NCBI Official Synonym Symbols: COX4; COXIV; COX4-1
• NCBI Protein Information: cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX IV-1; cytochrome c oxidase polypeptide IV
• UniProt Protein Name: Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• UniProt Gene Name: COX4I1
• UniProt Synonym Gene Names: COX4; COX IV-1
• UniProt Entry Name: COX41_HUMAN