Rabbit COX42 Polyclonal Antibody | anti-COX4I2 antibody

COX42 Antibody

Cat. Num. AAA286966

• Gene Names: COX4I2; COX4; COX4B; COX4-2; COX4L2; COXIV-2; dJ857M17.2
• Reactivity: Human, mouse, rat
• Applications: Immunofluorescence, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: COX42, Antibody; COX42 Antibody; Cytochrome c oxidase subunit 4 isoform 2; mitochondrial; Cytochrome c oxidase subunit IV isoform 2; COX IV-2; COX4I2; COX4L2; anti-COX4I2 antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Clonality: Polyclonal
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.09% (W/V) sodium azide and 50% glycerol.
• Sequence Positions: 55-80
• Sequence Length: 171

• Applicable Applications for anti-COX4I2 antibody: IF (Immunofluorescence), WB (Western Blot)
• Function: Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol- cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV,CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunbit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
• Cellular Location: Mitochondrion inner membrane {ECO:0000250|UniProtKB:P00423}; Single-pass membrane protein {ECO:0000250|UniProtKB:P00423}
• Tissue Location: Highly expressed in lung.
• Preparation and Storage: Store at -20°C

IF (Immunofluorescence)

(Immunofluorescence analysis of COS7 cells, using COX42 antibody.)

WB (Western Blot)

(Western blot analysis of lysate from human skeletal muscle tissue lysate,using COX42 Antibody(AAA286966). AAA286966 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug.)

Related Product Information for anti-COX4I2 antibody

This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Product Categories/Family for anti-COX4I2 antibody

Cancer; Metabolism; Signal Transduction

References

Huettemann M.,et al.Gene 267:111-123(2001).
Deloukas P.,et al.Nature 414:865-871(2001).
Shteyer E.,et al.Am. J. Hum. Genet. 84:412-417(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 17999526
• NCBI GeneID: 84701
• NCBI Accession #: NP_115998.2
• NCBI GenBank Nucleotide #: NM_032609.2
• UniProt Accession #: Q96KJ9; Q6GTF4; Q9H0Z4
• Molecular Weight: 20 kDa
• NCBI Official Full Name: cytochrome c oxidase subunit 4 isoform 2, mitochondrial
• NCBI Official Synonym Full Names: cytochrome c oxidase subunit IV isoform 2 (lung)
• NCBI Official Symbol: COX4I2
• NCBI Official Synonym Symbols: COX4; COX4B; COX4-2; COX4L2; COXIV-2; dJ857M17.2
• NCBI Protein Information: cytochrome c oxidase subunit 4 isoform 2, mitochondrial
• UniProt Protein Name: Cytochrome c oxidase subunit 4 isoform 2, mitochondrial
• UniProt Gene Name: COX4I2
• UniProt Synonym Gene Names: COX4L2; COX IV-2
• UniProt Entry Name: COX42_HUMAN