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WB (Western Blot)
(Anti-COX5B rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:13 kDa)
Rabbit anti-Human COX5B Polyclonal Antibody | anti-COX5B antibody
Anti-COX5B Antibody, Rabbit Polyclonal
Gene Names
COX5B; COXVB
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
COX5B, Antibody; Anti-COX5B Antibody, Rabbit Polyclonal; COX5B Antibody, Rabbit PAb, Antigen Affinity Purified; cytochrome c oxidase subunit Vb; Anti-COXVB Antibody; anti-COX5B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human COX5B
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-COX5B antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human COX5B protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human COX5B (rh COX5B). COX5B specific IgG was purified by Human COX5B affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-COX5B rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:13 kDa)
WB (Western Blot)
(Anti-COX5B rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:13 kDa)
IP (Immunoprecipitation)
(COX5B was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-COX5B rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-COX5B rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 14 kDaObserved band size: 14 kDa)
IP (Immunoprecipitation)
(COX5B was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-COX5B rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-COX5B rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 14 kDaObserved band size: 14 kDa)
IHC (Immunohiostchemistry)
(Immunochemical staining of human COX5B in human kidney with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human COX5B in human kidney with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human COX5B in human liver with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human COX5B in human liver with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-COX5B antibody
Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial-and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme.
References
Romero N. et al., 1990, Histochemistry. 94 (2): 211-5. Lomax MI. et al., 1991, Genomics. 10 (1): 1-9. Beauchemin A M. et al., 2001, Brain Res Bull. 56 (3-4): 285-97.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
129
NCBI Official Full Name
Cytochrome c oxidase subunit 5B, mitochondrial
NCBI Official Synonym Full Names
cytochrome c oxidase subunit Vb
NCBI Official Symbol
COX5B
NCBI Official Synonym Symbols
COXVB
NCBI Protein Information
cytochrome c oxidase subunit 5B, mitochondrial; cytochrome c oxidase polypeptide VB, mitochondrial
UniProt Protein Name
Cytochrome c oxidase subunit 5B, mitochondrial
UniProt Gene Name
COX5B
UniProt Entry Name
COX5B_HUMAN
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Product Notes
The COX5B cox5b (Catalog #AAA255833) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-COX5B Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COX5B can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the COX5B cox5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COX5B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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