IF (Immunofluorescence)
(AAA322726 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA322726) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
Rabbit anti-Human, Mouse COX6A2 Polyclonal Antibody | anti-COX6A2 antibody
COX6A2 Antibody
Gene Names
COX6A2; COX6AH; COXVIAH
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Synonyms
COX6A2, Antibody; COX6A2 Antibody; COX VIa M; COX6AH; COXVIAH; Cytochrome c oxidase polypeptide VIa heart; Cytochrome c oxidase subunit 6A2 mitochondrial; Cytochrome c oxidase subunit VIA muscle; Cytochrome c oxidase subunit VIa polypeptide 2; MS411; anti-COX6A2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
COX6A2 antibody detects endogenous levels of total COX6A2
Purity/Purification
Peptide affinity purification
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
97
Applicable Applications for anti-COX6A2 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human COX6A2, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20 degree C.
IF (Immunofluorescence)
(AAA322726 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA322726) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA322726 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA322726) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
WB (Western Blot)
(Western blot analysis of COX6A2 using COLO205 whole cell lysates)
WB (Western Blot)
(Western blot analysis of COX6A2 using COLO205 whole cell lysates)
IHC (Immunohistochemistry)
(AAA322726 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA322726 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-COX6A2 antibody
Function: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Similarity: Belongs to the cytochrome c oxidase subunit 6A family.
Similarity: Belongs to the cytochrome c oxidase subunit 6A family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11 kDa
NCBI Official Full Name
cytochrome c oxidase subunit 6A2, mitochondrial
NCBI Official Synonym Full Names
cytochrome c oxidase subunit 6A2
NCBI Official Symbol
COX6A2
NCBI Official Synonym Symbols
COX6AH; COXVIAH
NCBI Protein Information
cytochrome c oxidase subunit 6A2, mitochondrial
UniProt Protein Name
Cytochrome c oxidase subunit 6A2, mitochondrial
UniProt Gene Name
COX6A2
UniProt Synonym Gene Names
COX6A; COX6AH; COXVIAH; COX VIa-M
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Product Notes
The COX6A2 cox6a2 (Catalog #AAA322726) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The COX6A2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's COX6A2 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the COX6A2 cox6a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COX6A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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